Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer.

Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Research Abstract Details 

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Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Abstract Text:

Hisashi Tanaka,Yi Cao,Donald A Bergstrom,Charles Kooperberg,Stephen J Tapscott,Meng-Chao Yao,

Amplification of large chromosomal regions (gene amplification) is a common somatic alteration in human cancer cells and often is associated with advanced disease. A critical event initiating gene amplification is a DNA double-strand break (DSB), which is immediately followed by the formation of a large DNA palindrome. Large DNA palindromes are frequent and nonrandomly distributed in the genomes of cancer cells and facilitate a further increase in copy number. Although the importance of the formation of large DNA palindromes as a very early event in gene amplification is widely recognized, it is not known how a DSB is resolved to form a large DNA palindrome and whether any local DNA structure determines the location of large DNA palindromes. We show here that intrastrand annealing following a DNA double-strand break leads to the formation of large DNA palindromes and that DNA inverted repeats in the genome determine the efficiency of this event. Furthermore, in human Colo320DM cancer cells, a DNA inverted repeat in the genome marks the border between amplified and nonamplified DNA. Therefore, an early step of gene amplification is a regulated process that is facilitated by DNA inverted repeats in the genome.

Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Publishing Authors By Initials

H Tanaka,Y Cao,DA Bergstrom,C Kooperberg,SJ Tapscott,MC Yao,

For similar neoplasms research abstracts see: neoplasms research

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Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 1993-2002

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 22

MONTH: 01

YEAR: 2007

Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Keywords Mesh Terms:

KEYWORDS: Neoplasms

MESH TERMS: genetics

Chemical & Substance for Abstract: Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer.

AFFILIATION: Fred Hutchinson Cancer Reserach Center, Seattle, WA 98109, USA. tanakah@ccf.org

Country: United States

AGENCY: United States NIGMS

GRANT: R01GM 26210

ACRONYM: GM

MEDLINETA: Mol Cell Biol

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