Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics.

Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics. Research Abstract Details 

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Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics. Abstract Text:

Kenichi Matsuda,Mayumi Nishi,Hisamitsu Takaya,Natsuko Kaku,Mitsuhiro Kawata,Kenichi Matsuda,Mayumi Nishi,Hisamitsu Takaya,Natsuko Kaku,Mitsuhiro Kawata,

We analyzed the intranuclear dynamics of estrogen receptor alpha (ERalpha) and progesterone receptor (PR)-A/B labeled with different spectral variants of green fluorescent protein (GFP) in living cells. The distribution of ERalpha and PR-A/B were changed from a diffuse to discrete pattern after the addition of both ligands, but the extent of discrete cluster formation of PR-A/B was lower than that of ERalpha. The nuclear areas where PR-A/B were accumulated were colocalized with the cluster of ERalpha, suggesting that cross-talk in the transcriptional regulation occurred in the loci. Fluorescence recovery after photobleaching (FRAP) analysis revealed that the mobility of PR-A/B was hastened by the coexistence of ERalpha, while the mobility of ERalpha was not changed by the coexistence of PR-A/B. Cluster formation was correlated with the nuclear matrix binding, because nuclear matrix binding capacity was also lower in PR-A/B than ERalpha. By ATP-depletion from the cells, most of ERalpha and PR-A/B were bound to the nuclear matrix and their mobilities were extinguished both in the absence and presence of ligand. Fluorescent protein (FP) tagged nuclear matrix component protein (NuMA), which was colocalized with ERalpha and PR-A/B, showed ATP-dependent rapid exchange in the nucleus. These results indicate that the mobility of ERalpha and PR-A/B is associated with the dynamics of the nuclear matrix. J. Cell. Biochem. 103: 136-148, 2008. (c) 2007 Wiley-Liss, Inc.

Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics. Publishing Authors By Initials

K Matsuda,M Nishi,H Takaya,N Kaku,M Kawata,K Matsuda,M Nishi,H Takaya,N Kaku,M Kawata,

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Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cellular biochemistry

VOLUME: 103

Page Numbers: 136-48

Journal Abbreviation: J. Cell. Biochem.

ISSN: 0730-2312

DAY: 1

MONTH: Jan

YEAR: 2008

Intranuclear mobility of estrogen receptor alpha and progesterone receptors in association with nuclear matrix dynamics. Information

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LANGUAGE: eng

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AFFILIATION: Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.

Country: United States

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MEDLINETA: J Cell Biochem

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