Intranasal immunization against influenza virus using polymeric particles.

Intranasal immunization against influenza virus using polymeric particles. Research Abstract Details 

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Intranasal immunization against influenza virus using polymeric particles. Abstract Text:

D Lemoine,M Deschuyteneer,F Hogge,V Préat,

The aim of this study was to evaluate the potential of poly(D,L-lactide-co-glycolide) nano-and microspheres, with a mean diameter of 220 nm and 8 microm, respectively, to enhance the nasal and systemic immune responses against influenza virus antigen. High encapsulation levels of antigen were achieved in all cases. Neither the molecular weight nor the antigenicity of the entrapped antigen were affected by the encapsulation procedure. Following nasal immunization, the nasal washes IgA and the serum IgG responses were evaluated. With the soluble antigen, relatively high immune responses were observed. With nanospheres, nasal washes IgA levels were significantly lower (p<0.01) and serum IgG levels were not significantly different (p>0.05) from those obtained with the soluble antigen. With microspheres, both nasal washes IgA and serum IgG levels were significantly lower (p<0.01 and <0.05, respectively) as compared to the levels found for the soluble antigen. In addition, fluorescent microspheres administered intranasally failed to reach the nasal-associated lymphoid tissue (NALT). This lack of particle uptake by NALT and the high immunogenicity of the antigen used in this study, could explain the absence of enhancement of the immune responses by the polymeric particles.

Intranasal immunization against influenza virus using polymeric particles. Publishing Authors By Initials

D Lemoine,M Deschuyteneer,F Hogge,V Préat,

For similar therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research abstracts see: therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research

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Intranasal immunization against influenza virus using polymeric particles. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 10

Page Numbers: 805-25

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1999

Intranasal immunization against influenza virus using polymeric particles. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Intranasal immunization against influenza virus using polymeric particles. Keywords Mesh Terms:

KEYWORDS: Vaccination

MESH TERMS: methods

Chemical & Substance for Abstract: Intranasal immunization against influenza virus using polymeric particles. Information

Substance Name: Lactic Acid

Registry Number: 50-21-5

Grant and Affiliation Information for Intranasal immunization against influenza virus using polymeric particles.

AFFILIATION: Université catholique de Louvain, Unité de Pharmacie Galénique, Brussels, Belgium.

Country: NETHERLANDS

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ACRONYM:

MEDLINETA: J Biomater Sci Polym Ed

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