Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin.

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Abstract Text:

Qing Deng,Yue Zhang,Joseph T Barbieri,Qing Deng,Yue Zhang,Joseph T Barbieri,

Pseudomonas aeruginosa ExoS is a bifunctional type III cytotoxin that disrupts Ras- and Rho-signaling pathways in mammalian cells. A hydrophobic region (residues 51-77, termed the membrane localization domain) targets ExoS to the plasma membrane (PM) and late endosomes of host cells. In the current study, metabolic inhibitors and dominant-negative proteins that disrupt known vesicle-trafficking pathways were used to define the intracellular trafficking of ExoS. Release of ExoS from PM was independent of dynamin and ADP ribosylation factor 6 but inhibited by methyl-beta-cyclodextrin, a cholesterol-depleting reagent, and perinuclear localization of ExoS was disrupted by nocodazole. p50 dynamitin, a dynein inhibitor partially disrupted perinuclear localization of ExoS. Methyl-beta-cyclodextrin and nocodazole inhibited the ability of type-III-delivered ExoS to ADP-ribosylated Golgi/endoplasmic reticulum-resident Ras. Methyl-beta-cyclodextrin also relocated ExoS from the perinuclear region to the PM, indicating that ExoS can cycle through anterograde as well as through retrograde trafficking pathways. These findings show that ExoS endocytosis is cholesterol dependent, and it utilizes host microtubules, for intracellular trafficking. Understanding how type III cytotoxins enter and traffic within mammalian cells may identify new targets for therapeutic intervention of gram-negative bacterial pathogens.

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Publishing Authors By Initials

Q Deng,Y Zhang,JT Barbieri,Q Deng,Y Zhang,JT Barbieri,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Traffic (Copenhagen, Denmark)

VOLUME: 8

Page Numbers: 1331-45

Journal Abbreviation: Traffic

ISSN: 1398-9219

DAY: 20

MONTH: 08

YEAR: 2007

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100939340

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin.

AFFILIATION: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.

Country: Denmark

AGENCY: United States NIAID

GRANT: AI31062

ACRONYM: AI

MEDLINETA: Traffic

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin Related Publications

• A DENTAL STUDENT LOOKS AT THE PROFESSION.
• Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags.
• Antinociceptive activity of the N-methyl-D-aspartate receptor antagonist N-(2-Indanyl)-glycinamide hydrochloride (CHF3381) in experimental models of inflammatory and neuropathic pain.
• Association of botulinum neurotoxin serotypes a and B with synaptic vesicle protein complexes.
• Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae).
• Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.
• Chromosome fragility in cattle with chronic enzootic haematuria.
• Comparison of operative and conservative treatment of fractures of the lower end of the femur involving the knee.
• Developmental origins of health and disease (DOHaD).
• Effects of cardiac resynchronization therapy on insulin-like growth factor-1 in patients with advanced heart failure.
• Factors associated with infant and adolescent mortality.
• Fat graft myringoplasty after unsuccessful tympanic membrane repair.
• Immunization with the cysteine proteinase Ldccys1 gene from Leishmania (Leishmania) chagasi and the recombinant Ldccys1 protein elicits protective immune responses in a murine model of visceral leishmaniasis.
• Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.
• Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin.
• Local and systemic control in Ewing's sarcoma of the femur treated with chemotherapy, and locally by radiotherapy and/or surgery.
• Mechanism of substrate recognition by botulinum neurotoxin serotype A.
• Multiple pocket recognition of SNAP25 by botulinum neurotoxin serotype E.
• Multivariate classification and modeling in surface water pollution estimation.
• PSYCHOLOGY IN DENTAL PRACTICE.
• Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS.
• Racial inequalities and perinatal health in the southeast region of Brazil.
• Subunit Vaccine against the Seven Serotypes of Botulism.
• THYROGLOBULIN ANTIBODIES IN LEPROSY.
• The mortality impact of the August 2003 heat wave in France: Investigating the 'harvesting' effect and other long-term consequences.
• Why are the rates of cesarean section in Brazil higher in more developed cities than in less developed ones?
• [EVALUATION OF VARIOUS ENZYMATIC INDICES AND THE JIRGL TEST IN DIFFERENTIATION BETWEEN HEPATOCELLULAR AND CHOLOSTATIC JAUNDICE. II. APPLICATION OF DISCRIMINATING ANALYSES.]
• [Not Available.]
• [Not Available.]
• [Not Available.]