Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1.

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Abstract Text:

Wusheng Yan,Lynn Spruce,Michael M Rosenblatt,Thomas R Kleyman,Ronald C Rubenstein,

The A663T polymorphism of the alpha-subunit of the human epithelial sodium channel (hENaC) increases the functional and surface expression of alphabetagamma-hENaC in Xenopus laevis oocytes, and the context of this residue in the COOH terminus of alpha-hENaC is important for this effect. Query of a phosphoprotein database suggested that the alpha-T663 residue of hENaC might be a substrate for phosphorylation by casein kinase 1 (CK1). We tested the hypotheses that phosphorylation of alpha-T663-hENaC by CK1 would regulate the increased functional and surface expression of alpha-T663-hENaC vs. alpha-A663-hENaC in oocytes. General inhibition of CK1 with IC261 decreased the functional and surface expression of alpha-T663-hENaC, but not alpha-A663-hENaC. This decrease in alpha-T663-hENaC functional expression resulted from reduced delivery of alpha-T663-hENaC to the oocyte membrane. IC261 also inhibited the functional expression of alpha-T692-mENaC and a chimeric m(1-678)/h(650-669)alpha-T663, mbetagamma ENaC, but not alpha-A692-mENaC or m(1-678)/h(650-669)alpha-A663, mbetagamma ENaC. These data suggest that additional residues outside of the alpha-hENaC COOH terminus are important for modulation of alpha-T663-hENaC trafficking by CK1. Overexpression of CK1alpha did not alter functional expression of alpha-T663-hENaC. In contrast, modest overexpression of CK1delta enhanced, whereas higher levels of CK1delta overexpression inhibited alpha-T663-hENaC functional expression. CK1 did not phosphorylate the COOH terminus of either alpha-T663-hENaC or alpha-A663-hENaC in vitro. These data suggest that CK1, and perhaps specifically CK1delta, regulates the intracellular trafficking of the alpha-A663T functional polymorphism of hENaC indirectly by altering the rate of alpha-T663-hENaC biosynthesis and/or delivery to the plasma membrane.

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Publishing Authors By Initials

W Yan,L Spruce,MM Rosenblatt,TR Kleyman,RC Rubenstein,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research

PUBMED ID PMID:

MEDLINE DATE:

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Renal physiology

VOLUME: 293

Page Numbers: F868-76

Journal Abbreviation: Am. J. Physiol. Renal Physiol.

ISSN: 0363-6127

DAY: 27

MONTH: 06

YEAR: 2007

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901990

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Keywords Mesh Terms:

KEYWORDS: Xenopus laevis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1. Information

Substance Name: Casein Kinase I

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1.

AFFILIATION: Division of Pulmonary Medicine, The Children's Hospital of Philadelphia, PA, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK-58046

ACRONYM: DK

MEDLINETA: Am J Physiol Renal Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1 Related Publications

• A comprehensive molecular phylogeny of the starlings (Aves: Sturnidae) and mockingbirds (Aves: Mimidae): congruent mtDNA and nuclear trees for a cosmopolitan avian radiation.
• A spontaneously precipitable protein in human sera, with particular reference to the diagnosis of polyarteritis nodosa.
• Abnormal regulatory interactions of I148T-CFTR and the epithelial Na+ channel in Xenopus oocytes.
• Appropriateness of the decision to transfer nursing facility residents to the hospital.
• Dlx-dependent and -independent regulation of olfactory bulb interneuron differentiation.
• Dlx1 and Dlx2 control neuronal versus oligodendroglial cell fate acquisition in the developing forebrain.
• Does lowering of blood glucose improve cardiovascular morbidity and mortality?
• Effect of the substitution of muscle actin-specific subdomain 1 and 2 residues in yeast actin on actin function.
• Eustachian tube bypass.
• Frequency of going outdoors predicts long-range functional change among ambulatory frail elders living at home.
• Induction of p38MAPK and HSP27 phosphorylation in pemphigus patient skin.
• Institutional leadership and faculty response: fostering professionalism at the University of Pennsylvania School of Medicine.
• Intracellular trafficking of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel is modulated by casein kinase 1.
• Laparoscopic adjustable gastric banding at a U.S. center with up to 3-year follow-up.
• MR imaging and the evaluation of extracranial carotid stenosis.
• Passage of chronic wasting disease prion into transgenic mice expressing Rocky Mountain elk (Cervus elaphus nelsoni) PrPC.
• Patterning of frontal cortex subdivisions by Fgf17.
• Practical procedures in minor gynecology.
• Protein Biomarker Identification in the CSF of Patients With CNS Lymphoma.
• Recent advances in diagnosis and treatment of office gynecology.
• Regulatory interactions of N1303K-CFTR and ENaC in Xenopus oocytes: evidence that chloride transport is not necessary for inhibition of ENaC.
• Review: Collaborative care was effective for depression in primary care in the short and longer term.
• Siblings' coping strategies and mental health services: a national study of siblings of persons with schizophrenia.
• Site of intubation and duration of mechanical ventilation in pediatric status asthmaticus.
• Targeted therapy for cystic fibrosis: cystic fibrosis transmembrane conductance regulator mutation-specific pharmacologic strategies.
• The Segond fracture of the proximal tibia: a small avulsion that reflects major ligamentous damage.
• The evolution of changes in primary care delivery underlying the Veterans Health Administration's quality transformation.
• The evolution of cooperative breeding; is there cheating?
• The yield, reliability, and validity of a postal survey for screening community-dwelling older people.
• Workmen's compensation in the Ryukyus.