Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Abstract Text:

Yue Zhang,Qing Deng,Joseph T Barbieri,

ExoS (453 amino acids) is a bi-functional type III cytotoxin produced by Pseudomonas aeruginosa. Residues 96-219 include the Rho GTPase-activating protein (RhoGAP) domain, and residues 234-453 include the 14-3-3-dependent ADP-ribosyltransferase domain. Earlier studies also identified an N-terminal domain (termed the membrane localization domain) that comprises residues 51-77 and includes a novel leucine-rich motif that targets ExoS to the perinuclear region of cultured cells. There is limited information on how ExoS or other type III cytotoxins enter and target intracellular host proteins. Type III-delivered ExoS localized to both plasma membrane and perinuclear region, whereas ExoS(DeltaMLD) was localized to the cytosol. Plasma membrane localization of ExoS was transient and had a half-life of approximately 20 min. Type III-delivered ExoS co-immunoprecipitated 14-3-3 proteins and Rab9, Rab6, and Rab5. Immunofluorescence experiments showed that ExoS colocalized with Rab9, Rab6, and Rab5. Fluorescent energy transfer was detected between ExoS and 14-3-3 proteins but not between ExoS and Rabs proteins. Together, these results indicate that type III-delivered ExoS localizes on the host endosomes and utilizes multiple pathways to traffic from the plasma membrane to the perinuclear region of intoxicated host cells.

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Publishing Authors By Initials

Y Zhang,Q Deng,JT Barbieri,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rab gtp-binding proteins: rab5 gtp-binding proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rab gtp-binding proteins: rab5 gtp-binding proteins research

PUBMED ID PMID:

MEDLINE DATE:

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 13022-32

Journal Abbreviation:

ISSN: 0021-9258

DAY: 20

MONTH: 02

YEAR: 2007

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Keywords Mesh Terms:

KEYWORDS: rab5 GTP-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles. Information

Substance Name: rab5 GTP-Binding Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.

AFFILIATION: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI030162

ACRONYM: AI

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles Related Publications

• A DENTAL STUDENT LOOKS AT THE PROFESSION.
• Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags.
• Antinociceptive activity of the N-methyl-D-aspartate receptor antagonist N-(2-Indanyl)-glycinamide hydrochloride (CHF3381) in experimental models of inflammatory and neuropathic pain.
• Association of botulinum neurotoxin serotypes a and B with synaptic vesicle protein complexes.
• Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae).
• Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.
• Chromosome fragility in cattle with chronic enzootic haematuria.
• Comparison of operative and conservative treatment of fractures of the lower end of the femur involving the knee.
• Developmental origins of health and disease (DOHaD).
• Effects of cardiac resynchronization therapy on insulin-like growth factor-1 in patients with advanced heart failure.
• Factors associated with infant and adolescent mortality.
• Fat graft myringoplasty after unsuccessful tympanic membrane repair.
• Immunization with the cysteine proteinase Ldccys1 gene from Leishmania (Leishmania) chagasi and the recombinant Ldccys1 protein elicits protective immune responses in a murine model of visceral leishmaniasis.
• Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.
• Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin.
• Local and systemic control in Ewing's sarcoma of the femur treated with chemotherapy, and locally by radiotherapy and/or surgery.
• Mechanism of substrate recognition by botulinum neurotoxin serotype A.
• Multiple pocket recognition of SNAP25 by botulinum neurotoxin serotype E.
• Multivariate classification and modeling in surface water pollution estimation.
• PSYCHOLOGY IN DENTAL PRACTICE.
• Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS.
• Racial inequalities and perinatal health in the southeast region of Brazil.
• Subunit Vaccine against the Seven Serotypes of Botulism.
• THYROGLOBULIN ANTIBODIES IN LEPROSY.
• The mortality impact of the August 2003 heat wave in France: Investigating the 'harvesting' effect and other long-term consequences.
• Why are the rates of cesarean section in Brazil higher in more developed cities than in less developed ones?
• [EVALUATION OF VARIOUS ENZYMATIC INDICES AND THE JIRGL TEST IN DIFFERENTIATION BETWEEN HEPATOCELLULAR AND CHOLOSTATIC JAUNDICE. II. APPLICATION OF DISCRIMINATING ANALYSES.]
• [Not Available.]
• [Not Available.]
• [Not Available.]