Intracellular calcium waves in bone cell networks under single cell nanoindentation.

Intracellular calcium waves in bone cell networks under single cell nanoindentation. Research Abstract Details 

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Intracellular calcium waves in bone cell networks under single cell nanoindentation. Abstract Text:

X Edward Guo,Erica Takai,Xingyu Jiang,Qiaobing Xu,George M Whitesides,James T Yardley,Clark T Hung,Eugene M Chow,Thomas Hantschel,Kevin D Costa,

In this study, bone cells were successfully cultured into a micropatterned network with dimensions close to that of in vivo osteocyte networks using microcontact printing and self-assembled monolyers (SAMs). The optimal geometric parameters for the formation of these networks were determined in terms of circle diameters and line widths. Bone cells patterned in these networks were also able to form gap junctions with each other, shown by immunofluorescent staining for the gap junction protein connexin 43, as well as the transfer of gap-junction permeable calcein-AM dye. We have demonstrated for the first time, that the intracellular calcium response of a single bone cell indented in this bone cell network, can be transmitted to neighboring bone cells through multiple calcium waves. Furthermore, the propagation of these calcium waves was diminished with increased cell separation distance. Thus, this study provides new experimental data that support the idea of osteocyte network memory of mechanical loading similar to memory in neural networks.

Intracellular calcium waves in bone cell networks under single cell nanoindentation. Publishing Authors By Initials

XE Guo,E Takai,X Jiang,Q Xu,GM Whitesides,JT Yardley,CT Hung,EM Chow,T Hantschel,KD Costa,

For similar cells: connective tissue cells: osteoblasts research abstracts see: cells: connective tissue cells: osteoblasts research

PUBMED ID PMID:

MEDLINE DATE:

Intracellular calcium waves in bone cell networks under single cell nanoindentation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular & cellular biomechanics : MCB

VOLUME: 3

Page Numbers: 95-107

Journal Abbreviation:

ISSN: 1556-5297

DAY: 3

MONTH: Sep

YEAR: 2006

Intracellular calcium waves in bone cell networks under single cell nanoindentation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101253756

Intracellular calcium waves in bone cell networks under single cell nanoindentation. Keywords Mesh Terms:

KEYWORDS: Osteoblasts

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Intracellular calcium waves in bone cell networks under single cell nanoindentation. Information

Substance Name: fluorexon

Registry Number: 1461-15-0

Grant and Affiliation Information for Intracellular calcium waves in bone cell networks under single cell nanoindentation.

AFFILIATION: Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA. ed.guo@columbia.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM065364

ACRONYM: GM

MEDLINETA: Mol Cell Biomech

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