Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels.

Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Research Abstract Details 

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Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Abstract Text:

Takashi Sonobe,Tadakatsu Inagaki,David C Poole,Yutaka Kano,

Although the accumulation of intracellular calcium ions ([Ca(2+)](i)) is associated with muscle damage, little is known regarding the temporal profile of muscle [Ca(2+)](i) under in vivo conditions, and, specifically, the effects of different contraction types [e.g., isometric (ISO); eccentric (ECC)] on [Ca(2+)](i) remain to be determined. The following hypotheses were tested. 1) For 90 min at rest, an in vivo vs. in vitro preparation would better maintain initial [Ca(2+)](i). 2) Compared with ISO, ECC contractions (50 contractions, 10 sets, 5-min interval) would lead to a greater increase of [Ca(2+)](i). 3) Elevated [Ca(2+)](i) during ECC would be reduced or prevented by the stretch-activated ion channel blockers streptomycin and gadolinium (Gd(3+)). Spinotrapezius muscles of Wistar rats were exteriorized (in vivo) or excised (in vitro). [Ca(2+)](i) was evaluated by loading the muscle with fura 2-AM using fluorescence imaging. [Ca(2+)](i) rose progressively beyond 40 min at rest under in vitro but not in vivo conditions during the 90-min protocol. In vivo [Ca(2+)](i) increased more rapidly during ECC (first set) than ISO (fifth set) (P < 0.05 vs. precontraction values). The peak level of [Ca(2+)](i) was increased by 21.5% (ISO) and 42.8% (ECC) after 10 sets (both P < 0.01). Streptomycin and Gd(3+) abolished the majority of [Ca(2+)](i) increase during ECC (69 and 86% reduction, respectively; P < 0.01 from peak [Ca(2+)](i) of ECC). In conclusion, in vivo quantitative analyses demonstrated that ECC contractions elevate [Ca(2+)](i) significantly more than ISO contractions and that stretch-activated channels may play a permissive role in this response.

Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Publishing Authors By Initials

T Sonobe,T Inagaki,DC Poole,Y Kano,

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Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Regulatory, integr

VOLUME: 294

Page Numbers: R1329-37

Journal Abbreviation: Am. J. Physiol. Regul. Integr.

ISSN: 0363-6119

DAY: 16

MONTH: 01

YEAR: 2008

Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Information

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LANGUAGE: eng

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AFFILIATION: Depts. of Applied Physics and Chemistry, Univ. of Electro-Communications, Chofu, Tokyo 1828585, Japan. kano@pc.uec.ac.jp).

Country: United States

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MEDLINETA: Am J Physiol Regul Integr Comp

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