Interplasmid transposition demonstrates piggyBac mobility in vertebrate species.

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Research Abstract Details 

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Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Abstract Text:

Neil F Lobo,Tresa S Fraser,John A Adams,Malcolm J Fraser,

The piggyBac transposon is an extremely versatile helper-dependent vector for gene transfer and germ line transformation in a wide range of invertebrate species. Analyses of genome sequencing databases have identified piggyBac homologues among several sequenced animal genomes, including the human genome. In this report we demonstrate that this insect transposon is capable of transposition in primate cells and embryos of the zebrafish, Danio rerio. piggyBac mobility was demonstrated using an interplasmid transposition assay that has consistently predicted the germ line transformation capabilities of this mobile element in several other species. Both transfected COS-7 primate cells and injected zebrafish embryos supported the helper-dependent movement of tagged piggyBac element between plasmids in the characteristic cut-and-paste, TTAA target-site specific manner. These results validate piggyBac as a valuable tool for genetic analysis of vertebrates.

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Publishing Authors By Initials

NF Lobo,TS Fraser,JA Adams,MJ Fraser,

For similar animals: chordata: vertebrates: fishes: cypriniformes: cyprinidae: zebrafish research abstracts see: animals: chordata: vertebrates: fishes: cypriniformes: cyprinidae: zebrafish research

PUBMED ID PMID:

MEDLINE DATE:

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genetica

VOLUME: 128

Page Numbers: 347-57

Journal Abbreviation: Genetica

ISSN: 0016-6707

DAY: 3

MONTH: 12

YEAR: 2007

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370740

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Keywords Mesh Terms:

KEYWORDS: Zebrafish

MESH TERMS: genetics

Chemical & Substance for Abstract: Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Information

Substance Name: DNA Transposable Elements

Registry Number: 0

Grant and Affiliation Information for Interplasmid transposition demonstrates piggyBac mobility in vertebrate species.

AFFILIATION: Department of Biological Sciences, Center for Tropical Diseases Research and Training, University of Notre Dame, PO Box 369, Notre Dame, IN 46556-0369, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: R01 AI48561

ACRONYM: AI

MEDLINETA: Genetica

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