Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative.

Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Research Abstract Details 

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Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Abstract Text:

Nobuhisa Umeki,Toshiaki Mitsui,Yutaka Koike,Shinsaku Maruta,

A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which was an intermolecular cross-linked product of motor domains. In contrast, no cross-linking was observed in the absence of nucleotides. For the motor domain of mouse brain kinesin and skeletal muscle myosin subfragment-1, no such intermolecular photo cross-linking by Bz(2)-epsilonATP was observed. Our results indicate that Bz(2)-epsilonATP acts unusually as a photoreactive crosslinker to detect conformational changes in K16MD induced by nucleotide binding resulting in the formation of dimers.

Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Publishing Authors By Initials

N Umeki,T Mitsui,Y Koike,S Maruta,

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Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 831-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2006

Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative. Information

Substance Name: Kinesin

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Intermolecular cross-linking of a novel rice Kinesin k16 motor domain with a photoreactive ATP derivative.

AFFILIATION: Laboratories of Plant and Microbial Genome Control, Graduate School of Science and Technology, Niigata University, Niigata 950-2181.

Country: Japan

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MEDLINETA: J Biochem

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