Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells.

Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Research Abstract Details 

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Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Abstract Text:

Arianne L Theiss,Tracy S Obertone,Didier Merlin,Shanthi V Sitaraman,

Prohibitin (PHB) is a highly conserved protein that has multiple functions in the cell. We recently demonstrated that PHB plays an important role in combating oxidative stress and its expression is down-regulated in human and animal models of inflammatory bowel disease. Little is known regarding the regulation of PHB expression in intestine or other tissues. In this study we examined the regulation of PHB expression in intestinal epithelial cells using the model cell line Caco2-BBE. We successfully cloned the 1192-bp human PHB promoter region and identified the transcription start site 1594 bp upstream from the translation start site due to an intervening intron. We show that the acute phase cytokine interleukin-6 (IL-6) increases PHB protein and mRNA abundance and induces PHB promoter activation. The IL-6 response element site in the PHB promoter is required for maximal basal promoter activity and responsiveness to IL-6. IL-6 also increases binding of nuclear proteins to the IL-6 response element in the PHB promoter that are supershifted by a STAT3 antibody. Both basal promoter activity and IL-6 responsiveness are attenuated by signal transducer and activator of transcription 3 short interference RNA, suggesting that signal transducer and activator of transcription 3 mediates PHB activity by IL-6. Confirming these in vitro results, IL-6(-/-) mice exhibit reduced PHB expression in the colon compared with wild-type mice. These results suggest that IL-6 modulates PHB expression in cultured intestinal epithelial cells and in the intestine in vivo.

Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Publishing Authors By Initials

AL Theiss,TS Obertone,D Merlin,SV Sitaraman,

For similar genetic structures: genome: genome components: genes: gene components: regulatory elements, transcriptional: transcription initiation site research abstracts see: genetic structures: genome: genome components: genes: gene components: regulatory elements, transcriptional: transcription initiation site research

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Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 12804-12

Journal Abbreviation:

ISSN: 0021-9258

DAY: 26

MONTH: 02

YEAR: 2007

Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Keywords Mesh Terms:

KEYWORDS: Transcription Initiation Site

MESH TERMS: physiology

Chemical & Substance for Abstract: Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells. Information

Substance Name: prohibitin

Registry Number: 0

Grant and Affiliation Information for Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells.

AFFILIATION: Division of Digestive Diseases, Department of Medicine, Emory University, Atlanta, Georgia 30322, USA. atheiss@emory.edu

Country: United States

AGENCY: United States NIDDK

GRANT: R24-DK064399

ACRONYM: DK

MEDLINETA: J Biol Chem

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