Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations.

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Abstract Text:

Lixin Wang,Shanthi Srinivasan,Arianne L Theiss,Didier Merlin,Shanthi V Sitaraman,

Keratin 8 (K8) and keratin-18 (K18) are the major intermediate filament proteins in the intestinal epithelia. The regulation and function of keratin in the intestinal epithelia is largely unknown. In this study we addressed the role and regulation of K8 and K18 expression by interleukin 6 (IL-6). Caco2-BBE cell line and IL-6 null mice were used to study the effect of IL-6 on keratin expression. Keratin expression was studied by Northern blot, Western blot, and confocal microscopy. Paracellular permeability was assessed by apical-to-basal transport of a fluorescein isothiocyanate dextran probe (FD-4). K8 was silenced using the small interfering RNA approach. IL-6 significantly up-regulated mRNA and protein levels of K8 and K18. Confocal microscopy showed a reticular pattern of intracellular keratin localized to the subapical region after IL-6 treatment. IL-6 also induced serine phosphorylation of K8. IL-6 decreased paracellular flux of FD-4 compared with vehicle-treated monolayers. K8 silencing abolished the decrease in paracellular permeability induced by IL-6. Administration of dextran sodium sulfate (DSS) significantly increased intestinal permeability in IL-6-/- mice compared with wild type mice given DSS. Collectively, our data demonstrate that IL-6 regulates the colonic expression of K8 and K18, and K8/K18 mediates barrier protection by IL-6 under conditions where intestinal barrier is compromised. Thus, our data uncover a novel function of these abundant cytoskeletal proteins, which may have implications in intestinal disorders such as inflammatory bowel disease wherein barrier dysfunction underlies the inflammatory response.

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Publishing Authors By Initials

L Wang,S Srinivasan,AL Theiss,D Merlin,SV Sitaraman,

For similar natural sciences: chemistry: chemistry, physical: permeability research abstracts see: natural sciences: chemistry: chemistry, physical: permeability research

PUBMED ID PMID:

MEDLINE DATE:

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 8219-27

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 9

MONTH: 01

YEAR: 2007

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Keywords Mesh Terms:

KEYWORDS: Permeability

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations. Information

Substance Name: Keratins

Registry Number: 68238-35-7

Grant and Affiliation Information for Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations.

AFFILIATION: Division of Digestive Diseases, Department of Medicine, Emory University, Atlanta, Georgia 30322, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK06411

ACRONYM: DK

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations Related Publications

• A2B Adenosine Receptor Gene Deletion Attenuates Murine Colitis.
• Blockade of adenosine A(2B) receptors ameliorates murine colitis.
• Electrolytic preparation of azo-dyes.
• Epithelial-derived fibronectin expression, signaling, and function in intestinal inflammation.
• Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations.
• Interleukin-6 transcriptionally regulates prohibitin expression in intestinal epithelial cells.
• Matrix metalloproteinase-9 regulates MUC-2 expression through its effect on goblet cell differentiation.
• Matrix metalloproteinases in inflammatory bowel disease: boon or a bane?
• MicroRNAs: small molecules with big promise.
• Prohibitin protects against oxidative stress in intestinal epithelial cells.
• Purinergic receptors in gastrointestinal inflammation.