Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.

Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors. Research Abstract Details 

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Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors. Abstract Text:

Yuji Shimizu,Kenji Matsumoto,Yoshimichi Okayama,Kentaro Sakai,Toshitaka Maeno,Tatsuo Suga,Toru Miura,Shinji Takai,Masahiko Kurabayashi,Hirohisa Saito,Yuji Shimizu,Kenji Matsumoto,Yoshimichi Okayama,Kentaro Sakai,Toshitaka Maeno,Tatsuo Suga,Toru Miura,Shinji Takai,Masahiko Kurabayashi,Hirohisa Saito,

Although IL-3 is commonly used for culture of human progenitor-derived mast cells together with Stem cell factor (SCF) and IL-6, the effect of IL-3 on human mast cell differentiation has not been well elucidated. Human bone marrow CD34+ progenitors were cultured for up to 12 weeks in the presence of rhSCF and rhIL-6 either with rhIL-3 (IL-3 (+)) or without rhIL-3 (IL-3 (-)) for the initial 1-week of culture. Total cell number increased at 2 weeks in IL-3 (+), as compared to IL-3 (-), but changes in the appearance of mast cells were delayed. When IL-3 was present for the initial 1-week culture, granules looked more mature with IL-3 than without IL-3. However, tryptase and chymase contents, and surface antigen expression (CD18, CD51, CD54, and CD117) were not altered by IL-3. Surface expression and mRNA level of FcepsilonRIalpha and histamine release by crosslinking of FcepsilonRIalpha did not differ from one preparation to the next. GeneChip analysis revealed that no significant differences were observed between IL-3 (+) and IL-3 (-) cells either when inactivated or activated by aggregation of FcepsilonRIalpha. These findings indicate that initial incubation of human bone marrow CD34+ progenitors with IL-3 does not affect the differentiation of mast cells.

Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors. Publishing Authors By Initials

Y Shimizu,K Matsumoto,Y Okayama,K Sakai,T Maeno,T Suga,T Miura,S Takai,M Kurabayashi,H Saito,Y Shimizu,K Matsumoto,Y Okayama,K Sakai,T Maeno,T Suga,T Miura,S Takai,M Kurabayashi,H Saito,

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Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Immunological investigations

VOLUME: 37

Page Numbers: 1-17

Journal Abbreviation: Immunol. Invest.

ISSN: 0882-0139

DAY: 24

MONTH: 01

YEAR: 2008

Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors. Information

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LANGUAGE: eng

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AFFILIATION: Department of Respiratory Medicine, National Hospital Organization, Takasaki Hospital, Takasaki, Japan.

Country: United States

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MEDLINETA: Immunol Invest

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