Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation.

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Abstract Text:

Anna Shkoda,Pedro A Ruiz,Hannelore Daniel,Sandra C Kim,Gerhard Rogler,R Balfour Sartor,Dirk Haller,

BACKGROUND & AIMS: The initiation of endoplasmic reticulum (ER)-mediated stress responses in intestinal epithelial cells (IEC) may contribute to the pathogenesis of chronic intestinal inflammation. The aim of the study was to use functional epithelial cell proteomics to characterize anti-inflammatory mechanisms of interleukin 10 (IL-10). METHODS: Primary IEC were isolated from Enterococcus faecalis-monoassociated IL-10-deficient (IL-10-/-) and wild-type mice to perform 2D-gel sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. In addition, IEC from 6 patients with active Crohn's disease, ulcerative colitis, and sigmoid diverticulitis as well as noninflamed controls were purified. Molecular protective mechanisms of IL-10 were characterized in tumor necrosis factor (TNF)-stimulated IL-10 receptor (IL-10R) reconstituted epithelial cells. RESULTS: Primary IEC from IL-10-/- mice as well as inflammatory bowel disease patients revealed increased expression levels of the glucose-regulated ER stress protein (grp)-78 under conditions of chronic inflammation. Consistent with the observation that TNF induced ER stress responses through grp-78 redistribution from the ER lumen to the cytoplasmic IkappaB kinase complex, grp-78 knockdown completely abolished TNF-induced nuclear factor-kappaB RelA phosphorylation in epithelial cell cultures. Interestingly, IL-10 inhibited grp-78 protein and messenger RNA expression in IL-10R reconstituted IEC. Chromatin immunoprecipitation analysis and immunofluorescence microscopy revealed that IL-10-mediated p38 signaling inhibited TNF-induced recruitment of the ER-derived activating transcription factor (ATF)-6 to the grp-78 promoter likely through the blockade of ATF-6 nuclear translocation. CONCLUSIONS: Primary IEC from inflamed IL-10-/- mice and inflammatory bowel disease patients revealed activated ER stress responses in the intestinal epithelium. IL-10 inhibits inflammation-induced ER stress response mechanisms by modulating ATF-6 nuclear recruitment to the grp-78 gene promoter.

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Publishing Authors By Initials

A Shkoda,PA Ruiz,H Daniel,SC Kim,G Rogler,RB Sartor,D Haller,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

PUBMED ID PMID:

MEDLINE DATE:

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gastroenterology

VOLUME: 132

Page Numbers: 190-207

Journal Abbreviation: Gastroenterology

ISSN: 0016-5085

DAY: 21

MONTH: 10

YEAR: 2006

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374630

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation.

AFFILIATION: Else-Kroener-Fresenius Center for Experimental Nutritional Medicine, Technical University of Munich, 85350 Freising-Weihenstephan, Germany.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK 53347

ACRONYM: DK

MEDLINETA: Gastroenterology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation Related Publications

• A phase 1 study of fixed dose rate gemcitabine and irinotecan in patients with advanced pancreatic and biliary cancer.
• Bacteria- and host-derived mechanisms to control intestinal epithelial cell homeostasis: implications for chronic inflammation.
• Changes in Ventilatory Threshold in Mitochondrial Myopathies following Cycle Training, Deconditioning and Strength Training: 2015: Board #85 May 31 2:00 PM - 3:30 PM.
• Chemical aspects of some of the newer insecticides.
• Clinical pharmacology of 1,4-butanediol and gamma-hydroxybutyrate after oral 1,4-butanediol administration to healthy volunteers.
• Dentin diffusion of HEMA released from etch-and-rinse and self-etch bonding systems.
• Gaze pursuit, 'attention pursuit' and their effects on cortical activations.
• Glucocorticoid hyper- and hypofunction: stress effects on cognition and aggression.
• Hearing loss assessed by universal newborn hearing screening--the new approach.
• In the Aftermath of Nature's Fury.
• Interleukin-10 blocked endoplasmic reticulum stress in intestinal epithelial cells: impact on chronic inflammation.
• Luggage and shipped goods.
• Marginal integrity of class V restorations: SEM versus dye penetration.
• More bad news about anti-inflammatory agents.
• Multimedia article. Entirely thoracoscopic pneumonectomy using the prone position: a new technique.
• NOS3 polymorphisms are associated with arterial stiffness in children with type 1 diabetes.
• Nailing down PKC isoform specificity in diabetic nephropathy two's company, three's a crowd.
• Optical coherence tomography findings in persistent diabetic macular edema: the vitreomacular interface.
• Optical efficiency of far-infrared photoconductors.
• Res ipsa loquitor.
• The activation of prefrontal cortical neurons in aggression--a double labeling study.
• The effect of surface-active agents on phagocytosis.
• [Arrhenoblastoma]
• [Cesarean section in a moribund woman (survival of the child following cesarean section in the mother with pulmonary fibrosis)]
• [Clinical studies on peroral therapy of diabetes with a sulfonyl-urea derivative.]
• [Comparative studies of two radioimmunoassays for the rapid determination of human chorionic somatomammotropine (HCS)]
• [Diabetic nephropathy-current concepts in early diagnosis and treatment of diabetic microvascular complications]
• [Not Available.]
• [Not Available.]
• [Regenerative therapy in nephrology. Repair or construction?]