Interlaced optical force-fluorescence measurements for single molecule biophysics.

Interlaced optical force-fluorescence measurements for single molecule biophysics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interlaced optical force-fluorescence measurements for single molecule biophysics. Abstract Text:

Ricardo R Brau,Peter B Tarsa,Jorge M Ferrer,Peter Lee,Matthew J Lang,

Combining optical tweezers with single molecule fluorescence offers a powerful technique to study the biophysical properties of single proteins and molecules. However, such integration into a combined, coincident arrangement has been severely limited by the dramatic reduction in fluorescence longevity of common dyes under simultaneous exposure to trapping and fluorescence excitation beams. We present a novel approach to overcome this problem by alternately modulating the optical trap and excitation beams to prevent simultaneous exposure of the fluorescent dye. We demonstrate the dramatic reduction of trap-induced photobleaching effects on the common single molecule fluorescence dye Cy3, which is highly susceptible to this destructive pathway. The extension in characteristic fluorophore longevity, a 20-fold improvement when compared to simultaneous exposure to both beams, prolongs the fluorescence emission to several tens of seconds in a combined, coincident arrangement. Furthermore, we show that this scheme, interlaced optical force-fluorescence, does not compromise the trap stiffness or single molecule fluorescence sensitivity at sufficiently high modulation frequencies. Such improvement permits the simultaneous measurement of the mechanical state of a system with optical tweezers and the localization of molecular changes with single molecule fluorescence, as demonstrated by mechanically unzipping a 15-basepair DNA segment labeled with Cy3.

Interlaced optical force-fluorescence measurements for single molecule biophysics. Publishing Authors By Initials

RR Brau,PB Tarsa,JM Ferrer,P Lee,MJ Lang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Interlaced optical force-fluorescence measurements for single molecule biophysics. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biophysical journal

VOLUME: 91

Page Numbers: 1069-77

Journal Abbreviation:

ISSN: 0006-3495

DAY: 28

MONTH: 04

YEAR: 2006

Interlaced optical force-fluorescence measurements for single molecule biophysics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

Interlaced optical force-fluorescence measurements for single molecule biophysics. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Interlaced optical force-fluorescence measurements for single molecule biophysics. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Interlaced optical force-fluorescence measurements for single molecule biophysics.

AFFILIATION: Department of Mechanical Engineering, Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, 02139, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biophys J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interlaced optical force-fluorescence measurements for single molecule biophysics Related Publications

• A comparison of serratus anterior muscle activation during a wall slide exercise and other traditional exercises.
• Accuracy of noninvasive quantification of brain NAA concentrations using PRESS sequence: verification in a swine model with external standard.
• Adaptive ventricular rate smoothing during atrial fibrillation: a pilot comparison study.
• Angiogenic growth factor levels in maternal and fetal blood: correlation with Doppler ultrasound parameters in pregnancies complicated by pre-eclampsia and intrauterine growth restriction.
• Bioinformatics and its impact on clinical research methods.
• Brain potentials in perception: picture complexity and emotional arousal.
• Cell volume regulatory mechanisms.
• Cognition and self-control: cognitive control of painful sensory input.
• Delayed diagnosis of splenic rupture following minor trauma: Beware of comorbid conditions.
• Hormone, cytokine, and nutritional regulation of sepsis-induced increases in atrogin-1 and MuRF1 in skeletal muscle.
• Interlaced optical force-fluorescence measurements for single molecule biophysics.
• Intoxication level and emotional response.
• Longitudinal in vivo reproducibility of cartilage volume and surface in osteoarthritis of the knee.
• Longitudinal relationship of work hours, mandatory overtime, and on-call to musculoskeletal problems in nurses.
• Medical conditions increasing the risk of chronic thromboembolic pulmonary hypertension.
• Novel fast semi-automated software to segment cartilage for knee MR acquisitions.
• Regulation of endocytic recycling of KCNQ1/KCNE1 potassium channels.
• Relationship of complex spike synchrony bands and climbing fiber projection determined by reference to aldolase C compartments in crus IIa of the rat cerebellar cortex.
• Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle.
• Server intervention: what chance in Australia?
• Should emergency physicians use B-type natriuretic peptide testing in patients with unexplained dyspnea?
• Suicidal erythrocyte death in sepsis.
• The CD1d-binding glycolipid alpha-galactosylceramide enhances humoral immunity to T-dependent and T-independent antigen in a CD1d-dependent manner.
• The measurement of harmful outcomes following drinking on licensed premises.
• [Evaluation of the follow up results of patients with cervical intraepithelial neoplasia III after surgical treatment]
• [Harmony, improvement and progress]
• [Present and future of basic studies on endometriosis]
• [Present and future of studies on endometriosis (part two)]
• [Study of sexual function in women after surgery for stress urinary incontinence]
• [The chick embryo chorioallantioc membrane as a model for in vivo research on anti-angiogenesis in endometriosis]