Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed.

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Abstract Text:

Liangwen Pan,Shuya Zhang,Litao Yang,Hermann Broll,Fenghua Tian,Dabing Zhang,

The qualitative event-specific polymerase chain reaction detection method of genetically modified (GM) RT73 rapeseed was developed based on the cloned 3' end flanking sequence of RT73 rapeseed integration. The specificity of the method for GM RT73 rapeseed was validated using several different GM rapeseed lines, GM maize lines, GM soybean line, non-GM rapeseed, and other non-GM crops. In this study, the developed method was validated through an interlaboratory study by 12 laboratories from 6 countries. The sensitivity of this method was evaluated using several mixed rapeseed meals with different GM RT73 rapeseed contents from 5.0 to 0.01% prepared by our laboratory. The evaluated results showed that all of the rapeseed endogenous reference high mobility group protein gene (HMG I/Y), figwort mosaic virus 35S (FMV 35S) promoter, and RT73 event-specific fragment could be detected from rapeseed samples at 0.1% (w/w) with a confidence level of more than 95%. All results from the 12 laboratories indicated that the developed method could be considered fit for the detection and identification of GM RT73 rapeseed.

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Publishing Authors By Initials

L Pan,S Zhang,L Yang,H Broll,F Tian,D Zhang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 90

Page Numbers: 1639-46

Journal Abbreviation:

ISSN: 1060-3271

DAY: 15

MONTH: 01

YEAR: 2008

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed.

AFFILIATION: Shanghai Entry-Exit Inspection & Quarantine Bureau, GMO Detection Laboratory, 1208 Minsheng Rd, Shanghai 200135, People's Republic of China. panlw888@126.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J AOAC Int

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed Related Publications

• A comparative study on the intercuspal occlusion among TMD patients, malocclusion patients and university students.
• Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
• Enhanced antioxidant and cytoprotective abilities of vitamin E succinate is associated with a rapid uptake advantage in rat hepatocytes and mitochondria.
• Intervention by azithromycin on bleomycin-induced lung injury in rats and its mechanisms.
• Method development of adsorbed stationary phase open tubular capillary electrochromatography.
• Supercritical fluid CO2 extraction of colchicine from colchicine Autumnele liliaceae.
• Vascular endothelial growth factor gene expression in patients' ischemic skeletal muscle with diabetic foot.
• [A case of hypodermiasis with empyema]
• [Analysis of hepatitis G virus genotypes in some areas of China]
• [Autologous dendritic cells eliciting cytotoxicity of bone marrow cells against chronic granulocytic leukemia]
• [Changes of human recombination bone morphogenetic protein-2 in bone and marrow in tail suspended rats]
• [Cloning of the mouse Doc-1R gene by genomic walking]
• [Construction of recombinant adenoviruses carrying human urokinase-type plasminogen activator and its expression in hepatic stellate cells in vitro]
• [Effect of inhibition of telomerase activity on cisplatin-induced apoptosis in K562 cells]
• [Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats]
• [Fatal complications in traffic accident injury]
• [Indomethacin induces apoptosis of K562 cells through activation of caspases and elevation of intracellular free calcium]
• [Infection and replication of hepatitis B virus and its clearance mechanism: an overview]
• [Mechanisms underlying dopaminergic neurotoxicity in ischemic cerebral damage]
• [Physiological and pathologic implications for zero-stress state of the esophagus]
• [Protein kinases and Alzheimer's disease]
• [Retroperitoneal laparoscopic unroofing of renal cyst: use of close method]
• [Risk of leukemia among medical X-ray workers in China between 1950 and 1995]
• [Studies on association between lipoprotein lipase gene polymorphisms of Pvu II site and hypertriglyceridemics in Chinese]
• [Study on automatic segmentation of color images applied to blood cells]
• [Study on high performance liquid chromatography/indirect photometric detection of fosfomycin with acridine as detection reagent]
• [Study on the application of number fluorescence density in detecting autoantibodies titer]
• [The effects of surface morphology of calcium phosphate ceramics on apatite formation in dynamic SBF]
• [Transforming growth factor beta(1) and bone morphogenetic protein 2 induce the differentiation of odontoblasts in vitro]
• [Vector propagation algorithm based ECG simulation of bundle branch block]