Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells.

Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells. Research Abstract Details 

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Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells. Abstract Text:

Bazarragchaa Damdinsuren,Hiroaki Nagano,Hiroshi Wada,Takehiro Noda,Javzandulam Natsag,Shigeru Marubashi,Atsushi Miyamoto,Yutaka Takeda,Koji Umeshita,Yuichiro Doki,Keizo Dono,Morito Monden,

Aim: Interferon (IFN)-alpha is a promising drug for the prevention and treatment of hepatocellular carcinoma (HCC). We reported that responders to IFN-alpha/5-fluorouracil combination therapy expressed higher IFN alpha receptor (IFNAR)2 in tumor. Herein we studied involvement of IFNARs in response to IFN-alpha in HCC cells. Methods: IFN-alpha sensitivity and expression of IFNARs were studied in six HCC cell lines (HuH7, PLC/PRF/5, HLE, HLF, HepG2, Hep3B) using growth-inhibitory and RT-PCR, Western blot assays. Short interfering RNAs (SiRNAs) against IFNAR1 and 2 were used to analyze the role of the IFNARs in IFN-alpha's effect and signal transduction. Results: The expressions of IFNAR1 and 2c mRNAs were higher in PLC/PRF/5 cells than those in other cell lines, and PLC/PRF/5 cells expressed abundant IFNAR2c on their cell membrane. When we examined the sensitivity of the HCC cell lines to the growth-inhibitory effect of IFN-alpha, PLC/PRF/5 exhibited a significant response, while the other cells were much more resistant. Knockdown of either IFNAR1 or 2 using siRNAs suppressed the IFN-alpha's signal transduction (2.5-fold), and decreased the growth-inhibitory effect (down by 69.9% and 67.3%). Conclusion: The results suggest that the expression of IFNAR1 and IFNAR2c independently are important for the antiproliferative effect of IFN-alpha in HCC cells.

Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells. Publishing Authors By Initials

B Damdinsuren,H Nagano,H Wada,T Noda,J Natsag,S Marubashi,A Miyamoto,Y Takeda,K Umeshita,Y Doki,K Dono,M Monden,

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Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Hepatology research : the official journal of the

VOLUME: 37

Page Numbers: 77-83

Journal Abbreviation: Hepatol. Res.

ISSN: 1386-6346

DAY: 15

MONTH: Jan

YEAR: 2007

Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 9711801

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Grant and Affiliation Information for Interferon alpha receptors are important for antiproliferative effect of interferon-alpha against human hepatocellular carcinoma cells.

AFFILIATION: Department of Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.

Country: Netherlands

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MEDLINETA: Hepatol Res

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