Interactions of elongation factor EF-P with the Escherichia coli ribosome.

Interactions of elongation factor EF-P with the Escherichia coli ribosome. Research Abstract Details 

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Interactions of elongation factor EF-P with the Escherichia coli ribosome. Abstract Text:

Hiroyuki Aoki,John Xu,Andrew Emili,John G Chosay,Ashkan Golshani,M Clelia Ganoza,Hiroyuki Aoki,John Xu,Andrew Emili,John G Chosay,Ashkan Golshani,M Clelia Ganoza,

EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site.

Interactions of elongation factor EF-P with the Escherichia coli ribosome. Publishing Authors By Initials

H Aoki,J Xu,A Emili,JG Chosay,A Golshani,MC Ganoza,H Aoki,J Xu,A Emili,JG Chosay,A Golshani,MC Ganoza,

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Interactions of elongation factor EF-P with the Escherichia coli ribosome. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The FEBS journal

VOLUME: 275

Page Numbers: 671-81

Journal Abbreviation: FEBS J.

ISSN: 1742-464X

DAY: 12

MONTH: 01

YEAR: 2008

Interactions of elongation factor EF-P with the Escherichia coli ribosome. Information

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LANGUAGE: eng

NlmUniqueID: 101229646

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AFFILIATION: University of Toronto, C.H. Best Institute, Canada.

Country: England

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MEDLINETA: FEBS J

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