Interactions of arsenic with human metallothionein-2.

Interactions of arsenic with human metallothionein-2. Research Abstract Details 

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Interactions of arsenic with human metallothionein-2. Abstract Text:

Mitsutoshi Toyama,Mitsuo Yamashita,Noriaki Hirayama,Yoshikatsu Murooka,

Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As(3+) binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

Interactions of arsenic with human metallothionein-2. Publishing Authors By Initials

M Toyama,M Yamashita,N Hirayama,Y Murooka,

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Interactions of arsenic with human metallothionein-2. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 132

Page Numbers: 217-21

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 2002

Interactions of arsenic with human metallothionein-2. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Interactions of arsenic with human metallothionein-2. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Matrix-Assisted Lase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interactions of arsenic with human metallothionein-2. Information

Substance Name: Metallothionein

Registry Number: 9038-94-2

Grant and Affiliation Information for Interactions of arsenic with human metallothionein-2.

AFFILIATION: Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-oka, Suita, Osaka 565-0871, Japan.

Country: Japan

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MEDLINETA: J Biochem

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