Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes.

Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Research Abstract Details 

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Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Abstract Text:

Y Yang,S Kuramitsu,Y Nakae,K Ikeda,K Hamaguchi,

The binding constants of alpha- and beta-GlcNAc to hen and turkey lysozymes [EC 3.2.1.17] were determined at various pH's using the method proposed by Ikeda and Hamaguchi (1975) J. Biochem. 77, 1-16). The pH dependence of the binding of beta-GlcNAc to hen lysozyme was essentially the same as that for turkey lysozyme. The pH dependence curves of the binding constants of beta-GlcNAc to hen and turkey lysozymes were interpreted in terms of the participation of Glu 35 (pK 6.0), Asp 52 (pK 3.5), Asp 48 (pK 4.5), and Asp 66 (pK 1.5). The binding constants of alpha-GlcNAc to hen and turkey lysozymes were the same below pH 3.5 but were different above this pH. The main participant residues in the binding of alpha-GlcNAc were Glu 35, Asp 48, and Asp 66 for hen lysozyme and Glu 35 and Asp 66 for turkey lysozyme. The results obtained here were well explained by the following assumptions: (1) above about pH 4, alpha-GlcNAc binds to hen lysozyme in both alpha- and beta-modes, which correspond to the binding orientation of alpha-GlcNAc and that of beta-GlcNAc, respectively, as determined by X-ray crystallographic studies, but it binds predominantly in the beta-mode below about pH 4, (2) beta-GlcNAc binds to hen and turkey lysozymes predominantly in the beta-mode above about pH 4 and in both alpha- and beta-modes below pH 4, and (3) alpha-GlcNAc binds to turkey lysozyme predominantly in the beta-mode over the whole pH range studied.

Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Publishing Authors By Initials

Y Yang,S Kuramitsu,Y Nakae,K Ikeda,K Hamaguchi,

For similar animals: chordata: vertebrates: birds: galliformes: turkeys research abstracts see: animals: chordata: vertebrates: birds: galliformes: turkeys research

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Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 80

Page Numbers: 425-34

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1976

Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Keywords Mesh Terms:

KEYWORDS: Turkeys

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interactions of alpha- and beta-N-acetyl-D-glucosamines with hen and turkey lysozymes. Information

Substance Name: Muramidase

Registry Number: EC 3.2.1.17

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Country: JAPAN

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MEDLINETA: J Biochem

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