Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats.

Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Research Abstract Details 

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Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Abstract Text:

Owen Richard Davies,Luca Pellegrini,

BRCA2 has an essential function in DNA repair by homologous recombination, interacting with RAD51 via short motifs in the middle and at the C terminus of BRCA2. Here, we report that a conserved 36-residue sequence of human BRCA2 encoded by exon 27 (BRCA2Exon27) interacts with RAD51 through the specific recognition of oligomerized RAD51 ATPase domains. BRCA2Exon27 binding stabilizes the RAD51 nucleoprotein filament against disassembly by BRC repeat 4. The protection is specific for RAD51 filaments formed on single-stranded DNA and is lost when BRCA2Exon27 is phosphorylated on Ser3291. We propose that productive recombination results from the functional balance between the different RAD51-binding modes of the BRC repeat and exon 27 regions of BRCA2. Our results further suggest a mechanism in which CDK phosphorylation of BRCA2Exon27 at the G2-M transition alters the balance in favor of RAD51 filament disassembly, thus terminating recombination.

Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Publishing Authors By Initials

OR Davies,L Pellegrini,

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Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 475-83

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9993

DAY: 10

MONTH: Jun

YEAR: 2007

Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Information

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LANGUAGE: eng

NlmUniqueID: 101186374

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Grant and Affiliation Information for Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats.

AFFILIATION: University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1GA, UK.

Country: United States

AGENCY: United Kingdom Wellcome T

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MEDLINETA: Nat Struct Mol Biol

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