Interaction of vinculin with the clathrin heavy chain.

Interaction of vinculin with the clathrin heavy chain. Research Abstract Details 

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Interaction of vinculin with the clathrin heavy chain. Abstract Text:

J L Fausser,E Ungewickell,J V Ruch,H Lesot,

To further document the interaction of vinculin with the clathrin heavy chain (CHC) which was observed by using gel overlay, co-sedimentation experiments were performed and attempts were made to localize the domains involved on both molecules. The binding properties of proteolytic fragments of vinculin were investigated after cleavage with V8 protease. Neither the isolated globular domain, nor the C-terminal rod domain were able to interact with the CHC. Either the interaction involved the portion of vinculin which links these two domains, or the region of vinculin mediating the interaction was present on one of the two major fragments, but the cleavage itself resulted in conformational changes which abolished the binding. The first hypothesis could be ruled out using alpha-chymotrypsin generated fragments of vinculin, suggesting that the native conformation of vinculin might play an important role. Proteolytic cleavage of CHC with trypsin demonstrated that the interaction with vinculin is mediated by the proximal or distal segment of the CHC. Presence of clathrin light chain (CLC) associated with the CHC did not affect its interaction with vinculin. Vinculin did not interact with the CLC.

Interaction of vinculin with the clathrin heavy chain. Publishing Authors By Initials

JL Fausser,E Ungewickell,JV Ruch,H Lesot,

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

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Interaction of vinculin with the clathrin heavy chain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 114

Page Numbers: 498-503

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1993

Interaction of vinculin with the clathrin heavy chain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Interaction of vinculin with the clathrin heavy chain. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interaction of vinculin with the clathrin heavy chain. Information

Substance Name: glutamyl endopeptidase

Registry Number: EC 3.4.21.19

Grant and Affiliation Information for Interaction of vinculin with the clathrin heavy chain.

AFFILIATION: Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.

Country: JAPAN

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MEDLINETA: J Biochem

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