Interaction of the ruthenium red cation with nucleic acid double helices.

Interaction of the ruthenium red cation with nucleic acid double helices. Research Abstract Details 

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Interaction of the ruthenium red cation with nucleic acid double helices. Abstract Text:

R L Karpel,M S Shirley,S R Holt,

The hexapositive complex cation ruthenium red very effectively stabilizes DNA and RNA double helices against thermal denaturation. In the presence of nucleic acid helices, this symmetric cation acquires an extrinsic CD spectrum near the wavelength of the dye's maximum absorbance. Competition experiments with single-stranded polyd(T) show this induced CD to be the result of selective binding to helical sites. The preferential affinity of ruthenium red for double helical binding sites is so great that it brings about biphasic absorbance- temperature profiles of polyd(A-T) at low [cation]: [polynucleotide phosphate]. The visible CD signal and fraction of helix melting at the upper transition increases with ruthenium red concentration until approximate charge neutrality is reached. These interactions, which have been studied in detail with the poly(U-U) helix as well as polyd(A-T), are likely largely electrostatic, since sufficient [NaCl] eliminates the bipliasic melting of polyd(A-T), renders the ultraviolet absorbance of poly (U) insensitive to ruthenium red, and abolishes the induced CD effects. The bipliasic melting of polyd(A-T) at intermediate [dye] is attributed to saturation of remaining double helical segments by cation migration from newly melted regions- Furthermore, virtually no change was observed in the induced CD upon melting through the first transition, whereas the effect is destroyed upon inciting through the second transition. A quantitative treatment of the data is used to obtain binding site size and association constant for the complex. The induced effect may prove useful in the exploration of exposed nucleic acid helical structure in such complex particles as nucleosomes or ribosomes.

Interaction of the ruthenium red cation with nucleic acid double helices. Publishing Authors By Initials

RL Karpel,MS Shirley,SR Holt,

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Interaction of the ruthenium red cation with nucleic acid double helices. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biophysical chemistry

VOLUME: 13

Page Numbers: 151-65

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ISSN: 0301-4622

DAY: 26

MONTH: Apr

YEAR: 1981

Interaction of the ruthenium red cation with nucleic acid double helices. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Interaction of the ruthenium red cation with nucleic acid double helices.

AFFILIATION: Department of Chemistry, University of Maryland Baltimore County, Calonsville, Maryland 21228, USA.

Country: Netherlands

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MEDLINETA: Biophys Chem

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