Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer.

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Abstract Text:

H Aoyagi,S Lee,T Kanmera,H Mihara,T Kato,

Six peptide fragments, SEP6-11, SEP6-15, SEP1-11, SEP1-15, SEP1-20, and SEP25-39, corresponding to the residues 6-11, 6-15, 1-11, 1-15, 1-20, and 25-39, respectively, of the extension peptide of cytochrome P-450(SCC) precursor were chemically synthesized by the solution method. CD spectra of the peptides indicated that SEP1-15 and SEP1-20, which inhibit the import of the precursor, held a random conformation in a Tris-HCl buffer and a partially ordered conformation (like alpha-helix or type II' beta-turn) in a buffer containing acidic liposomes. Slightly changed spectra were observed for SEP1-15 and SEP1-20 in the buffer containing neutral liposomes and in MeOH, respectively. Other peptides which show weak or no inhibitory effect had almost random conformations in these solvents. The hydrophobic moments of SEP1-15 and SEP1-20 when they take alpha-helical conformation are very small, suggesting that amphiphilic helical property is not always necessary for the import of the precursor, although partially ordered conformation seems to be required. The ability of SEP1-15 and SEP1-20 to induce leakage of carboxyfluorescein from the inside of dipalmitoyl-DL-alpha-phosphatidylcholine (DPPC) or DPPC-dipalmitoyl-DL-alpha-phosphatidylglycerol (3:1) vesicles was much greater than that of other peptides. The leakage induction ability of the peptides qualitatively parallels the degree of their inhibition of the import of the precursor. Perturbation of the membranes caused by the action of the peptides in their partially ordered form may be important for the import of the precursor into mitochondria.

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Publishing Authors By Initials

H Aoyagi,S Lee,T Kanmera,H Mihara,T Kato,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research

PUBMED ID PMID:

MEDLINE DATE:

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 102

Page Numbers: 813-20

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1987

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Keywords Mesh Terms:

KEYWORDS: Protein Conformation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer. Information

Substance Name: Cholesterol Side-Chain Cleavage Enzyme

Registry Number: EC 1.14.15.6

Grant and Affiliation Information for Interaction of synthetic fragments of the extension peptide of cytochrome P-450(SCC) precursor with phospholipid bilayer.

AFFILIATION: laboratory of Biochemistry, Faculty of Science, Kyushu University, Fukuoka.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interaction of synthetic fragments of the extension peptide of cytochrome P-450SCC precursor with phospholipid bilayer Related Publications

• A consideration of the characteristics of ABR on the basis of a differential equation model and the estimation of parameters of its solution.
• A novel obligate cultivation mutualism between damselfish and Polysiphonia algae.
• A possible relationship between the anti-cancer potency of photodynamic therapy using the novel photosensitizer ATX-s10-Na(II) and expression of the vascular endothelial growth factor in vivo.
• Bone morphogenetic protein 2 suppresses the transformed phenotype and restores actin microfilaments of human lung carcinoma A549 cells.
• Development of on-bed lower extremity exercise system for use with networked virtual space.
• Diverse glycosylation of MUC1 and MUC2: potential significance in tumor immunity.
• Dopamine induces a slow afterdepolarization in lateral amygdala neurons.
• Effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 combined with fibrous collagen membranes at subperiosteal sites.
• Effect of oviductal epithelial cells on fertilization of pig oocytes in vitro.
• Effect of recombinant human platelet-derived growth factor-BB and bone morphogenetic protein-2 application to demineralized dentin on early periodontal ligament cell response.
• Effects of hyaluronic acid on the development of 1- and 2-cell porcine embryos to the blastocyst stage in vitro.
• Glucocorticoid receptor activation is involved in producing abnormal phenotypes of single-prolonged stress rats: a putative post-traumatic stress disorder model.
• Grafted neural stem cells increase the life span and protect motoneurons in pmn mice.
• Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate.
• Helicobacter pylori cytotoxin-associated genotype and gastric precancerous lesions.
• Hematopoietic engraftment in recipients of unrelated donor umbilical cord blood is affected by the CD34+ and CD8+ cell doses.
• Identification and characterization of a dextranase gene of Streptococcus criceti.
• Influence of season on characteristics of epididymal and ejaculated semen in Meishan boars.
• Iron overload and cofactors with special reference to alcohol, hepatitis C virus infection and steatosis/insulin resistance.
• Isolated tumor cell in lateral lymph node has no influences on the prognosis of rectal cancer patients.
• Personalized medicine and proteomics: lessons from non-small cell lung cancer.
• Phase-matching properties for AgGaGeS4.
• Protease-catalyzed esterification of amino acid in water-miscible ionic liquid.
• Purpurin expression in the zebrafish retina during early development and after optic nerve lesion in adults.
• Reconstruction of central lacteals in the murine jejunum following ischemia-reperfusion injury.
• Testicular development in Chinese Meishan boars.
• Thyroid hormone status of newborns in relation to in utero exposure to PCBs and hydroxylated PCB metabolites.
• Transcutaneous fluorescent imaging with a depth-dependent point spread function.
• Treatment strategy for synchronous metastases of colorectal cancer: is hepatic resection after an observation interval appropriate?
• [Impression procedures for removable partial dentures.]