Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein.

Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Research Abstract Details 

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Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Abstract Text:

W J Shen,K Sridhar,D A Bernlohr,F B Kraemer,

Hormone-sensitive lipase (HSL) is a cytosolic neutral lipase that functions as the rate-limiting enzyme for the mobilization of free fatty acids in adipose tissue. By using the yeast two-hybrid system to examine the potential interaction of HSL with other cellular proteins, evidence is provided to demonstrate a direct interaction of HSL with adipocyte lipid-binding protein (ALBP), a member of the family of intracellular lipid-binding proteins that binds fatty acids, retinoids, and other hydrophobic ligands. The interaction was demonstrated in vitro by the binding of ALBP to HSL translated in vitro, to HSL in extracts of HSL overexpressing Chinese hamster ovary (CHO) cells, and to HSL in extracts of rat adipose tissue. Finally, the presence of ALBP was documented in immune complexes from rat adipose tissue immunoprecipitated with anti-HSL antibodies. The HSL-ALBP interaction was mapped to an N-terminal 300-aa region of HSL that is distinct from the C-terminal catalytic domain. These results suggest that HSL-derived fatty acids are bound by ALBP to facilitate intracellular trafficking of hydrophobic lipids.

Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Publishing Authors By Initials

WJ Shen,K Sridhar,DA Bernlohr,FB Kraemer,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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MEDLINE DATE:

Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 96

Page Numbers: 5528-32

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 11

MONTH: May

YEAR: 1999

Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein. Information

Substance Name: Sterol Esterase

Registry Number: EC 3.1.1.13

Grant and Affiliation Information for Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein.

AFFILIATION: Division of Endocrinology, Department of Medicine, Stanford University, Stanford, CA 94305-5103, USA.

Country: UNITED STATES

AGENCY: United States NIDDK

GRANT: DK 49705

ACRONYM: DK

MEDLINETA: Proc Natl Acad Sci U S A

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ACCESSION NUMBER: AF144756

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