Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins.

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Abstract Text:

Y Murakami,H Iwata,E Kitano,H Kitamura,Y Ikada,

Influence of various biomaterials on the complement system in serum has been intensively studied by many research groups, since activation of the complement pathway in vivo has been known to give rise to some pathological conditions, such as inflammation and anaphylaxis. Much effort has been devoted to develop new materials that do not activate or deteriorate the complement system. The present work is aimed at revealing the mode of reactions of anionic poly(2-acrylamido 2-methylpropane sulfonate) grafted on polystyrene bead (PAMPS-g-bead) with serum complement. Complement activity assay, determination of complement proteins levels, and immunoblot analysis were carried out for sera pretreated with PAMPS-g-beads. The results clearly showed that, when PAMPS-g-beads were incubated with serum, those beads adsorbed several complement proteins, i.e. C1q, factor D, factor P, C6, and C8, but the generation of activation fragments of complement components was not observed. Especially, factor D was most effectively removed from serum, resulting in potential inhibition of the alternative pathway. A larger amount of PAMPS-g-beads was needed to decrease the serum CH50 level. That may be caused by removal of C6. Although some polyanions, such as dextran sulfate, were reported to activate the complement system, the obtained results indicate that the PAMPS-g-bead is not an activator of the complement pathway, but acts as an adsorbent of complement components. One possible clinical application of the PAMPS-g-beads is adsorption of serum factor D by extracorporeal treatment of patients with renal failure with a high level of factor D, because the increased quantity of factor D in serum may cause consistent activation of the alternative pathway.

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Publishing Authors By Initials

Y Murakami,H Iwata,E Kitano,H Kitamura,Y Ikada,

For similar inorganic chemicals: acids: acids, noncarboxylic: sulfur acids: sulfonic acids research abstracts see: inorganic chemicals: acids: acids, noncarboxylic: sulfur acids: sulfonic acids research

PUBMED ID PMID:

MEDLINE DATE:

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 12

Page Numbers: 451-65

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2001

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Keywords Mesh Terms:

KEYWORDS: Sulfonic Acids

MESH TERMS: metabolism

Chemical & Substance for Abstract: Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins. Information

Substance Name: Complement System Proteins

Registry Number: 9007-36-7

Grant and Affiliation Information for Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins.

AFFILIATION: Institute for Frontier Medical Sciences, Kyoto University, Japan.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomater Sci Polym Ed

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interaction of poly2-acrylamido 2-methylpropane sulfonate-grafted polystyrene beads with cationic complement proteins Related Publications

• A bioabsorbable delivery system for antibiotic treatment of osteomyelitis. The use of lactic acid oligomer as a carrier.
• Alveolar bone regeneration using absorbable poly(L-lactide-co-varepsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblast growth factor.
• An immuno-isolative membrane capable of consuming cytolytic complement proteins.
• Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1.
• Controlled release of vascular endothelial growth factor by use of collagen hydrogels.
• Cross-linking of amniotic membranes.
• Depressed tuberculin sensitivity in Darier's disease.
• Depressed tuberculin sensitivity in porokeratosis.
• Dextran sulfate as a material for the preparation of a membrane for immunoisolation.
• Ectopic bone formation induced by biodegradable hydrogels incorporating bone morphogenetic protein.
• Encapsulation of mammalian cells into synthetic polymer membranes using least toxic solvents.
• Enzyme-catalyzed synthesis of a bioactive oligopeptide in nearly anhydrous solvents with polyethylene glycol-modified proteases.
• Fusogenic activity of various water-soluble polymers.
• In vivo chondrogenesis in collagen sponge sandwiched by perichondrium.
• Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor.
• Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins.
• Interaction of poly(styrene sulfonic acid) with the alternative pathway of the serum complement system.
• Interaction of poly(styrene sulfonic acid) with the classical pathway of the serum complement system.
• Letter: Hyperkeratosis lenticularis perstans.
• Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor.
• New biodegradable oligoesters for pharmaceutical application.
• New detachable balloon technique for traumatic carotid-cavernous sinus fistulae.
• Preparation of DNA-immobilized immunoadsorbent for treatment of systemic lupus erythematosus.
• Regeneration of extrahepatic bile duct -possibility to clinical application by recognition of the regenerative process.
• Regeneration of the inferior vena cava with a bioabsorbable polymer implant: a histological study.
• Soft tissue adhesive composed of modified gelatin and polysaccharides.
• Spatial gradient effects of 120 mT static magnetic field on endothelial tubular formation in vitro.
• Surface modification of silicone for percutaneous implantation.
• Treatment of a spontaneous carotid cavernous fistula using an electrodetachable microcoil.
• Vascularization into a porous sponge by sustained release of basic fibroblast growth factor.