Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules.

Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules. Research Abstract Details 

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Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules. Abstract Text:

Nobuo Terada,Nobuhiko Ohno,Sei Saitoh,George Seki,Masayuki Komada,Tatsuo Suzuki,Hisashi Yamakawa,Manoocher Soleimani,Shinichi Ohno,Nobuo Terada,Nobuhiko Ohno,Sei Saitoh,George Seki,Masayuki Komada,Tatsuo Suzuki,Hisashi Yamakawa,Manoocher Soleimani,Shinichi Ohno,Nobuo Terada,Nobuhiko Ohno,Sei Saitoh,George Seki,Masayuki Komada,Tatsuo Suzuki,Hisashi Yamakawa,Manoocher Soleimani,Shinichi Ohno,

Our recent studies demonstrated the localization of protein 4.1B, a member of the 4.1 skeletal membrane proteins, to the basolateral membranes of the S1-S2 renal proximal tubules. In the present studies, we investigated the presence of binding partners that could form a molecular complex with the 4.1B protein. Immunohistochemistry revealed the localization of p55, a membrane-associated guanylate kinase, and the sodium bicarbonate cotransporter1 (NBC1), to the basolateral membrane domain of S1-S2 in mouse renal proximal tubules. Using immunoprecipitation of kidney lysates with anti-p55 antibody, a positive band was blotted with anti-4.1B antibody. GST fusion proteins including the NBC1 and 4.1B regions were confirmed to bind with each other by electrophoresis after mixing. Both NBC1- and 4.1B-specific bands were detected in renal protein mixtures immunoprecipated by either anti-4.1B- or NBC1-specific antibodies. It is likely that NBC1, 4.1B, and p55 form a molecular complex in the basolateral membrane of the kidney S1-S2 proximal tubules. We propose that the 4.1B-containing membrane skeleton may play a role in regulating the Na(+) and HCO(3)(-) reabsorption in S1-S2 proximal tubules.

Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules. Publishing Authors By Initials

N Terada,N Ohno,S Saitoh,G Seki,M Komada,T Suzuki,H Yamakawa,M Soleimani,S Ohno,N Terada,N Ohno,S Saitoh,G Seki,M Komada,T Suzuki,H Yamakawa,M Soleimani,S Ohno,N Terada,N Ohno,S Saitoh,G Seki,M Komada,T Suzuki,H Yamakawa,M Soleimani,S Ohno,

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Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The journal of histochemistry and cytochemistry :

VOLUME: 55

Page Numbers: 1199-206

Journal Abbreviation: J. Histochem. Cytochem.

ISSN: 0022-1554

DAY: 21

MONTH: 08

YEAR: 2007

Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules. Information

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LANGUAGE: eng

NlmUniqueID: 9815334

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Grant and Affiliation Information for Interaction of Membrane Skeletal Protein, Protein 4.1B and p55, and Sodium Bicarbonate Cotransporter1 in Mouse Renal S1-S2 Proximal Tubules.

AFFILIATION: Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo City, Yamanashi 409-3898, Japan. nobuot@yamanashi.ac.jp.

Country: United States

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MEDLINETA: J Histochem Cytochem

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