Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality.

Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality. Research Abstract Details 

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Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality. Abstract Text:

Mandy Pereira,Penelope Mason,Roman J Szczesny,Leena Maddukuri,Sylwia Dziwura,Robert Jedrzejczak,Erin Paul,Andrzej Wojcik,Lien Dybczynska,Barbara Tudek,Ewa Bartnik,Jan Klysik,Vilhelm A Bohr,Piotr P Stepien,Mandy Pereira,Penelope Mason,Roman J Szczesny,Leena Maddukuri,Sylwia Dziwura,Robert Jedrzejczak,Erin Paul,Andrzej Wojcik,Lien Dybczynska,Barbara Tudek,Ewa Bartnik,Jan Klysik,Vilhelm A Bohr,Piotr P Stepien,

The SUV3 gene is present in all eukaryotes and encodes an RNA/DNA helicase which operates both in mitochondria and cell nuclei. To assess its function in mammals we generated a mouse mutant strain in which the 3' part of the SUV3 gene is disrupted. The mutated allele is a hypomorph transmitted from one generation to another at a frequency about 35% lower than expected while mice homozygous for the mutation die in utero before midgestation. Using ELISA binding assays we show that human SUV3 protein interacts with human WRN and BLM helicases. The binding to BLM protein was 10-fold stronger (with a K(d) of 0.5nM) than to WRN protein (K(d) of 5nM). Silencing of the SUV3 gene in the human cell line HeLa resulted in elevation of homologous recombination as measured by the frequency of sister chromatid exchange during mitotic cell division. These results indicate that the SUV3 protein is required in mammalian development and in somatic cells participates in genome maintenance through interaction with other genome fidelity housekeepers.

Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality. Publishing Authors By Initials

M Pereira,P Mason,RJ Szczesny,L Maddukuri,S Dziwura,R Jedrzejczak,E Paul,A Wojcik,L Dybczynska,B Tudek,E Bartnik,J Klysik,VA Bohr,PP Stepien,M Pereira,P Mason,RJ Szczesny,L Maddukuri,S Dziwura,R Jedrzejczak,E Paul,A Wojcik,L Dybczynska,B Tudek,E Bartnik,J Klysik,VA Bohr,PP Stepien,

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PUBMED ID PMID:

MEDLINE DATE: 2007 Nov-Dec

Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Mechanisms of ageing and development

VOLUME: 128

Page Numbers: 609-17

Journal Abbreviation: Mech. Ageing Dev.

ISSN: 0047-6374

DAY: 14

MONTH: 09

YEAR: 2007

Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality. Information

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LANGUAGE: eng

NlmUniqueID: 347227

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Grant and Affiliation Information for Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality.

AFFILIATION: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.

Country: Ireland

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MEDLINETA: Mech Ageing Dev

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