Interaction of human plasma fibronectin with alpha-elastin.

Interaction of human plasma fibronectin with alpha-elastin. Research Abstract Details 

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Interaction of human plasma fibronectin with alpha-elastin. Abstract Text:

S Harumiya,S K Jung,Y Sakano,D Fujimoto,

Because there are contradictory reports about the interaction of plasma fibronectin with elastin, we investigated the interaction in vitro. When human plasma was applied to an alpha-elastin-Sepharose column at 4 degrees C, the column-binding fraction contained fibronectin. When isolated plasma fibronectin was applied to the same column at 4 degrees C, most of the fibronectin bound to the column and was eluted with 1 M KBr. However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. The elevation of the surface hydrophobicity of plasma fibronectin at 4 degrees C was observed by hydrophobic chromatography, using alkyl-Sepharose columns. It seems that the binding of plasma fibronectin to alpha-elastin involves hydrophobic interaction, which is affected by temperature and possibly by other factors.

Interaction of human plasma fibronectin with alpha-elastin. Publishing Authors By Initials

S Harumiya,SK Jung,Y Sakano,D Fujimoto,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Interaction of human plasma fibronectin with alpha-elastin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 710-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1993

Interaction of human plasma fibronectin with alpha-elastin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Interaction of human plasma fibronectin with alpha-elastin. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Interaction of human plasma fibronectin with alpha-elastin. Information

Substance Name: Elastin

Registry Number: 9007-58-3

Grant and Affiliation Information for Interaction of human plasma fibronectin with alpha-elastin.

AFFILIATION: Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology.

Country: JAPAN

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MEDLINETA: J Biochem

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