Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy.

Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Research Abstract Details 

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Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Abstract Text:

M Onda,H Hayashi,T Mita,

The effect of the interaction of gramicidin (GA) with lysophosphatidylcholine (LPC) on the change in lipid structure upon heat incubation was revealed by differential scanning calorimetry (DSC) and fluorescence spectroscopy. DSC showed a large endothermic transition in both pure LPC micelles and GA-containing LPC micelles after prolonged heat incubation at 70 degrees C. To elucidate this behavior, fluorescence spectra of 1-anilinonaphthalene-8-sulfonate embedded in LPC micelles were measured. About 40% of the resultant LPC micelles was found to be transformed into the interdigitated gel structures after prolonged heat incubation. On the other hand, intrinsic fluorescence spectra of GA-containing LPC micelles caused a blue-shift of the emission maxima with incubation time, suggesting that tryptophans near the C-terminus of GA moved into a more apolar environment. In addition, GA-containing LPC micelles caused quenching of fluorescence with incubation time, due to the interaction between GA molecules. To determine the location of GA in LPC membranes, surface pressure was measured using the mixed monolayers composed of GA and LPC. The result suggests that GA molecule is localized by interdigitating the C-terminal part of adjacent to acyl chain of LPC.

Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Publishing Authors By Initials

M Onda,H Hayashi,T Mita,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 613-20

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 2001

Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy. Information

Substance Name: Gramicidin

Registry Number: 1405-97-6

Grant and Affiliation Information for Interaction of gramicidin with lysophosphatidylcholine as revealed by calorimetry and fluorescence spectroscopy.

AFFILIATION: Department of Environmental Sciences, Faculty of Science, Osaka Women's University, Daisen-cho, Sakai, Osaka 590-0035, Japan.

Country: Japan

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MEDLINETA: J Biochem

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