Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site.

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Abstract Text:

Pier Paolo D'Avino,Tetsuya Takeda,Luisa Capalbo,Wei Zhang,Kathryn S Lilley,Ernest D Laue,David M Glover,

Anillin, one of the first factors recruited to the cleavage site during cytokinesis, interacts with actin, myosin II and septins, and is essential for proper organization of the actomyosin contractile ring. We employed affinity-purification methodology coupled with mass spectrometry to identify Anillin-interacting molecules in Drosophila cells. We isolated several actin and myosin proteins, three of the five Drosophila septins and RacGAP50C (Tum), a component of the centralspindlin complex. Using drug and RNA interference (RNAi) treatments we established that F-actin is essential for Anillin cortical localization in prometaphase but not for its accumulation at the cleavage furrow after anaphase onset. Moreover, septins were not recruited to the cleavage site in cells in which Anillin was knocked down by RNAi, but localized to central-spindle microtubules, suggesting that septins travel along microtubules to interact with Anillin at the furrow. Finally, we demonstrate that RacGAP50C is necessary for Anillin accumulation at the furrow and that the two proteins colocalize in vivo and interact in vitro. Thus, in addition to its role in activating RhoA signalling, RacGAP50C also controls the proper assembly of the actomyosin ring by interacting with Anillin at the cleavage furrow.

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Publishing Authors By Initials

PP D'Avino,T Takeda,L Capalbo,W Zhang,KS Lilley,ED Laue,DM Glover,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cell science

VOLUME: 121

Page Numbers: 1151-8

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 18

MONTH: 03

YEAR: 2008

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 52457

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site.

AFFILIATION: Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Cell Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site Related Publications

• A novel VEGFR3 mutation causes Milroy disease.
• A technique for pulmonary segmental delineation.
• Amniotic fluid testosterone: relationship with cortisol and gestational age.
• Antenatal maternal stress and long-term effects on child neurodevelopment: how and why?
• Assessing the influence of scanner background noise on auditory processing. I. An fMRI study comparing three experimental designs with varying degrees of scanner noise.
• Assessing the influence of scanner background noise on auditory processing. II. An fMRI study comparing auditory processing in the absence and presence of recorded scanner noise using a sparse design.
• Changing cultures: enhancing mental health and wellbeing of refugee young people through education and training.
• Chemistry of 4-alkylaryloxenium ion "precursors": sound and fury signifying something?
• Chromosome fragile sites.
• Controlled inspiration depth reduces variance in breath-holding-induced BOLD signal.
• DSAS-6 organizes a tube-like centriole precursor, and its absence suggests modularity in centriole assembly.
• Effect of the hypocholesterolemic drug, AY 9944, on the absorption of retinol in the rat.
• Endogenous oxidized indoles share inhibitory potency against [3H]isatin binding in rat brain.
• Frontostriatal connectivity and its role in cognitive control in parent-child dyads with ADHD.
• Macular degeneration: types, causes, and possible interventions.
• Management of allergic sinusitis.
• Maternal stress during pregnancy predicts cognitive ability and fearfulness in infancy.
• Parental and adolescent smoking: does the association vary with gender and ethnicity?
• Poultry disease problems in Ontario.
• ROCK-II mediates colon cancer invasion via regulation of MMP-2 and MMP-13 at the site of invadopodia as revealed by multiphoton imaging.
• Reducing interscanner variability of activation in a multicenter fMRI study: controlling for signal-to-fluctuation-noise-ratio (SFNR) differences.
• Relationship between hypoxia, oxygen consumption and body temperature.
• Restricted field of view magnetic resonance imaging of a dynamic time series.
• Spatiotemporal patterns of neurogenesis in the appendicularian Oikopleura dioica.
• State mental health policy: critical priorities confronting state mental health agencies.
• The clinical and economic potential of hyperbaric oxygen therapy in the treatment of diabetic ulceration and other conditions.
• The personal librarian program:an evaluation of a cushing/whitney medical library outreach initiative.
• The role of steric effects in the direct mutagenicity of N-acyloxy-N-alkoxyamides.
• Three-dimensional spiral technique for high-resolution functional MRI.
• [Not Available.]