Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma.

Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Research Abstract Details 

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Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Abstract Text:

T Yamamoto,K Yamamoto,H Sinohara,

Urinary excretion of trypsin inhibitor increased after injection of a carcinogen, N-nitrosobis(2-oxopropyl)amine, into Syrian hamsters. Two inhibitors were purified to apparent homogeneity from urine collected during the course of the carcinogenesis experiment. Their complete amino acid sequences were determined by Edman degradation of the intact proteins and partially degraded fragments. One corresponded to a hamster liver cDNA clone that hybridized with human bikunin probe [Ide et al, (1994) Biochim, Biophys. Acta 1209, 286-292], except that the protein sequence lacked C-terminal serine and the other was trypstatin, the C-terminal half of the bikunin molecule. Three proteins containing covalently linked bikunin were also identified in pooled blood plasma. They were all dissociated into heavy and light chains by treatment with chondroitinase ABC or 50 mM NaOH, but not by heating at 100 degrees C in the presence of sodium dodecyl sulfate and dithiothreitol, N-terminal amino acid sequence analyses of the native chains and partially degraded fragments thereof revealed that these proteins are (i) human-type inter-alpha-trypsin inhibitor, consisting of heavy chains 1 and 2 and bikunin, (ii) bovine-type inter-alpha-trypsin inhibitor, consisting of heavy chains 2 and 3 and bikunin, and (iii) pre-alpha-trypsin inhibitor, consisting of heavy chain 3 and bikunin. Heterodimer of bikunin/heavy chain 1 or bikunin/heavy chain 2 was not detected. These results suggest that the composition, and hence function, of the inter-alpha-trypsin inhibitor family differs considerably from species to species.

Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Publishing Authors By Initials

T Yamamoto,K Yamamoto,H Sinohara,

For similar chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research abstracts see: chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research

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Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 120

Page Numbers: 145-52

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1996

Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Keywords Mesh Terms:

KEYWORDS: Trypsin Inhibitors

MESH TERMS: analysis

Chemical & Substance for Abstract: Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma. Information

Substance Name: Chondroitin Lyases

Registry Number: EC 4.2.2.-

Grant and Affiliation Information for Inter-alpha-trypsin inhibitor and its related proteins in Syrian hamster urine and plasma.

AFFILIATION: Department of Biochemistry, Kinki University School of Medicine, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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