IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT.

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Abstract Text:

Jeanne M Dichiara,Aras N Mattis,Jeffrey F Gardner,

CTnDOT is a Bacteroides conjugative transposon (CTn) that has facilitated the spread of antibiotic resistances among bacteria in the human gut in recent years. Although the integrase encoded by CTnDOT (IntDOT) carries the C-terminal set of conserved amino acids that is characteristic of the tyrosine family of recombinases, the reaction it catalyzes involves a novel step that creates a short region of heterology at the joined ends of the element during recombination. Also, in contrast to tyrosine recombinases, IntDOT catalyzes a reaction that is not site specific. To determine what types of contacts IntDOT makes with the DNA during excision and integration, we first developed an agarose gel-based assay for CTnDOT recombination, which facilitated the purification of the native IntDOT protein. The partially purified IntDOT was then used for DNase I footprinting analysis of the integration site attDOT and the excision sites attL and attR. Our results indicate that CTnDOT has five or six arm sites that are likely to be involved in forming higher-order nucleoprotein complexes necessary for synapsis. In addition, there are four core sites that flank the sites of strand exchange during recombination. Thus, despite the fact that the reaction catalyzed by IntDOT appears to be different from that typically catalyzed by tyrosine recombinases, the protein-DNA interactions required for higher-order structures and recombination appear to be similar.

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Publishing Authors By Initials

JM Dichiara,AN Mattis,JF Gardner,

For similar investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research abstracts see: investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research

PUBMED ID PMID:

MEDLINE DATE:

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 2692-701

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 2

MONTH: 02

YEAR: 2007

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Keywords Mesh Terms:

KEYWORDS: Polymerase Chain Reaction

MESH TERMS: genetics

Chemical & Substance for Abstract: IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT. Information

Substance Name: Deoxyribonuclease I

Registry Number: EC 3.1.21.1

Grant and Affiliation Information for IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT.

AFFILIATION: Department of Microbiology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-28717

ACRONYM: GM

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT Related Publications

• A witnessed short fall mimicking presumed shaken baby syndrome (inflicted childhood neurotrauma).
• CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges.
• Cardiopulmonary resuscitation in pregnancy.
• Characterization of a conjugative transposon integrase, IntDOT.
• Comparison of morphological effects of PGE2 and TGFbeta on osteoclastogenesis induced by RANKL in mouse bone marrow cell cultures.
• Cooperation peaks at intermediate disturbance.
• Differences in vascular reactivity between men and women.
• Discrimination of prostate cancer cells by reflection mode FTIR photoacoustic spectroscopy.
• Dynamic programming algorithms for comparing multineuronal spike trains via cost-based metrics and alignments.
• Embryo culture medium: which is the best?
• Fatal pneumococcal meningitis: failure of acute care focus of medical practice.
• Hypoxic regulation of Id-1 and activation of the unfolded protein response are aberrant in neuroblastoma.
• In vitro combination characterization of the new anticancer plant drug beta-elemene with taxanes against human lung carcinoma.
• Incurring greater health care costs: risk stratification of employees with bipolar disorder.
• IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT.
• Interaction of the Gifsy-1 Xis protein with the Gifsy-1 attP sequence.
• Large-scale mapping and validation of Escherichia coli transcriptional regulation from a compendium of expression profiles.
• Levo-tetrahydropalmatine inhibits cocaine's rewarding effects: experiments with self-administration and brain-stimulation reward in rats.
• Optical artefacts in transflection mode FTIR microspectroscopic images of single cells on a biological support: the effect of back-scattering into collection optics.
• Previous traumatic birth: an impetus for requested cesarean birth.
• Selective leukemic-cell killing by a novel functional class of thalidomide analogs.
• Surviving change: Can the ESRD provisions in the SCHIP create a level playing field?
• The bacteroides NBU1 integrase performs a homology-independent strand exchange to form a holliday junction intermediate.
• The core role of the nurse practitioner: practice, professionalism and clinical leadership.
• The effect of current cigarette smoking on calf muscle hemoglobin oxygen saturation in patients with intermittent claudication.
• The influence of obesity on arterial compliance in adult men and women.
• The relationship between arterial elasticity and metabolic syndrome features.
• Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene.
• Understanding systemic sclerosis through gene expression profiling.
• Vitamin D activates type A natriuretic peptide receptor gene transcription in inner medullary collecting duct cells.