Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages.

Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages. Research Abstract Details 

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Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages. Abstract Text:

Mareike Leffler,Thomas Hrach,Michael Stuerzl,Raymund E Horch,David N Herndon,Marc G Jeschke,Mareike Leffler,Thomas Hrach,Michael Stuerzl,Raymund E Horch,David N Herndon,Marc G Jeschke,Mareike Leffler,Thomas Hrach,Michael Stuerzl,Raymund E Horch,David N Herndon,Marc G Jeschke,

BACKGROUND: Insulin decreases the incidence of sepsis and improves mortality of critically ill patients. In endotoxemic as well as in thermally injured rats, insulin attenuates the systemic inflammatory response by decreasing the proinflammatory and increasing the antiinflammatory cascade. The aim of the present study was to determine the effects of insulin on cell survival, cell activity, apoptosis, and proinflammatory response in a human macrophage-like cell line (THP-1 cells) stressed with lipopolysaccharide (LPS). MATERIALS AND METHODS: Human macrophages were stressed with LPS and received either saline or insulin. Cell viability was analyzed by MTS, apoptosis was detected using JC-1 and terminal deoxynucleotidyl transferase-mediated nick end labeling-staining, and to elucidate on the signaling pathway, we used wortmannin as a phosphatidylinositol-3-kinase inhibitor. Tumor necrosis factor (TNF) and interleukin-1beta (IL-1beta) were measured to determine the effect of insulin on proinflammatory cytokine expression. RESULTS: Insulin caused a significant increase in cell viability and significantly reduced apoptosis in LPS-stimulated human macrophages in a dose-dependent manner. The antiapoptotic effect of insulin could be completely blocked with the addition of wortmannin. Insulin significantly decreased TNF and IL-1beta in endotoxemic human macrophages. CONCLUSIONS: Our results indicate that insulin exerts antiapoptotic effects and reduces the expression of proinflammatory cytokines in endotoxemic human macrophages. The antiapoptotic effects are mediated via the phospatidylinositol-3-kinase-pathway.

Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages. Publishing Authors By Initials

M Leffler,T Hrach,M Stuerzl,RE Horch,DN Herndon,MG Jeschke,M Leffler,T Hrach,M Stuerzl,RE Horch,DN Herndon,MG Jeschke,M Leffler,T Hrach,M Stuerzl,RE Horch,DN Herndon,MG Jeschke,

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Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of surgical research

VOLUME: 143

Page Numbers: 398-406

Journal Abbreviation: J. Surg. Res.

ISSN: 0022-4804

DAY: 20

MONTH: 06

YEAR: 2007

Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages. Information

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LANGUAGE: eng

NlmUniqueID: 376340

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Grant and Affiliation Information for Insulin attenuates apoptosis and exerts anti-inflammatory effects in endotoxemic human macrophages.

AFFILIATION: Department of Plastic and Hand Surgery, University Hospital Erlangen-Nürnberg, Erlangen, Germany.

Country: United States

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MEDLINETA: J Surg Res

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