Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector.

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Abstract Text:

Sarata C Sahu,Virgil Simplaceanu,Qingguo Gong,Nancy T Ho,Fang Tian,James H Prestegard,Chien Ho,

We present a nuclear magnetic resonance (NMR) study in solution of the structures of human normal hemoglobin (Hb A) in the deoxy or unligated form in the absence and presence of an allosteric effector, inositol hexaphosphate (IHP), using 15N-1H residual dipolar coupling (RDC) measurements. There are several published crystal structures for deoxyhemoglobin A (deoxy-Hb A), and it has been reported that the functional properties of Hb A in single crystals are different from those in solution. Carbonmonoxyhemoglobin A (HbCO A) can also be crystallized in several structures. Our recent RDC studies of HbCO A in the absence and presence of IHP have shown that the solution structure of this Hb molecule is distinctly different from its classical crystal structures (R and R2). To have a better understanding of the structure-function relationship of Hb A under physiological conditions, we need to evaluate its structures in both ligated and unligated states in solution. Here, the intrinsic paramagnetic property of deoxy-Hb A has been exploited for the measurement of RDCs using the magnetic-field dependence of the apparent one-bond 1H-15N J couplings. Our RDC analysis suggests that the quaternary and tertiary structures of deoxy-Hb A in solution differ from its recently determined high-resolution crystal structures. Upon binding of IHP, structural changes in deoxy-Hb A are also observed, and these changes are largely within the alpha1beta1 (or alpha2beta2) dimer itself. These new structural findings allow us to gain a deeper insight into the structure-function relationship of this interesting allosteric protein.

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Publishing Authors By Initials

SC Sahu,V Simplaceanu,Q Gong,NT Ho,F Tian,JH Prestegard,C Ho,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

PUBMED ID PMID:

MEDLINE DATE:

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemistry

VOLUME: 46

Page Numbers: 9973-80

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 11

MONTH: 08

YEAR: 2007

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: chemistry

Chemical & Substance for Abstract: Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Information

Substance Name: Hemoglobin A

Registry Number: 9034-51-9

Grant and Affiliation Information for Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector.

AFFILIATION: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.

Country: United States

AGENCY: United States NCRR

GRANT: S10RR-017815

ACRONYM: RR

MEDLINETA: Biochemistry

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector Related Publications

• Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction.
• Ageing and ART: a waste of time and money?
• An in vitro model of morphine withdrawal manifests the enhancing effect on human immunodeficiency virus infection of human T lymphocytes through the induction of substance P.
• Apigenin suppresses cancer cell growth through ERbeta.
• Bandgap-assisted surface-plasmon sensing.
• Characterization of mucins in human middle ear and Eustachian tube.
• Characterization of the mouse Muc3 membrane bound intestinal mucin 5' coding and promoter regions: regulation by inflammatory cytokines.
• Cloning and characterization of mouse intestinal MUC3 mucin: 3' sequence contains epidermal-growth-factor-like domains.
• Concurrent laparoscopic right hemicolectomy and ultra-low anterior resection with colonic J-pouch anal anastomosis for synchronous carcinoma.
• Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice.
• Developmental estrogen exposures predispose to prostate carcinogenesis with aging.
• Directing macromolecular conformation through halogen bonds.
• Directly measured insulin resistance and the assessment of clustered cardiovascular risks in hypertension.
• Does certificate of need affect cardiac outcomes and costs?
• Effects of lipid association on lomustine (CCNU) administered intracerebrally to syngeneic 36B-10 rat brain tumors.
• Expression of mucins in mucoid otitis media.
• Feasibility of weekly HIV drug delivery to enhance drug localization in lymphoid tissues based on pharmacokinetic models of lipid-associated indinavir.
• Health-seeking patterns among Chinese immigrant patients enrolled in the directly observed therapy program in New York City.
• High-resolution dynamic imaging of contrast agent uptake in a beating heart.
• MRI detection of macrophages labeled using micrometer-sized iron oxide particles.
• Methamphetamine-induced behavioral sensitization in mice: alterations in mu-opioid receptor.
• Natural killer cell inhibits human immunodeficiency virus replication in chronically infected immune cells.
• Orientation of deoxyhemoglobin at high magnetic fields: structural insights from RDCs in solution.
• Overexpression of mitochondrial superoxide dismutase in mice protects the retina from diabetes-induced oxidative stress.
• Repression of the hindbrain developmental program by Cdx factors is required for the specification of the vertebrate spinal cord.
• Spectroscopic evaluation of human tear lipids.
• Sulforaphane retards the growth of human PC-3 xenografts and inhibits HDAC activity in human subjects.
• Theoretical investigation of the mechanisms of reactions of H2CN and H2SiN with NO.
• Treatments of multi-invasive giant prolactinoma.
• Xanthohumol, a prenylflavonoid derived from hops induces apoptosis and inhibits NF-kappaB activation in prostate epithelial cells.