Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain.

Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Research Abstract Details 

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Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Abstract Text:

PURPOSE: To prospectively investigate the long-term effect of adeno-associated viral (AAV) vector-encoding vascular endothelial growth factor gene (VEGF) (AAV-VEGF) on left ventricular (LV) mass and volumes, as well as on regional contractility and circumferential strain, in a swine model of reperfused myocardial infarction. MATERIALS AND METHODS: All experimental procedures received approval from the institutional committee on animal research. Of 16 pigs subjected to reperfused myocardial infarction, six were treated, six were controls, and four died during the ischemic intervention. In six animals, cardiac-specific AAV-VEGF was injected into the periinfarcted and infarcted myocardium 1 hour after reperfusion. Magnetic resonance (MR) imaging was performed at 3 days and 8 weeks after infarction by using cine, tagged, and delayed enhancement (with gadoterate meglumine) sequences to measure global and regional LV function and infarct size. At postmortem examination, tissue samples stained with isolectin B4, Masson trichrome, and hematoxylin-eosin were used to characterize injured myocardium. Two-tailed Student t test was used for statistical analysis. RESULTS: Six treated animals showed no change in mean LV ejection fraction after 8 weeks (40.3%+/-0.9 [standard error of the mean] vs 41.0%+/-0.7) in contrast to a decrease measured in six control animals (41.4%+/-0.7 vs 36.1%+/-0.6, P<.001). AAV-VEGF improved wall thickening and circumferential strain in periinfarcted and remote myocardium. A greater reduction in gadoterate meglumine-enhanced infarct area was measured in treated animals (18.6%+/-1.5 of the LV mass at 3 days vs 9.8%+/-1.0 of the LV mass at 8 weeks, P<.001) compared with control animals (17.7%+/-2.0 vs 14.8%+/-1.0, P=.008). Findings at histopathologic evaluation indicated an increase in vascular density and a decrease in myocyte diameter in the periinfarcted myocardium of treated, compared with control, animals. CONCLUSION: Angiogenesis and arteriogenesis induced by VEGF genes improved regional myocardial strain and wall thickening and preserved ejection fraction after infarction.

Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Publishing Authors By Initials

For similar circulatory and respiratory physiology: cardiovascular physiology: cardiovascular physiologic processes: ventricular function: ventricular function, left research abstracts see: circulatory and respiratory physiology: cardiovascular physiology: cardiovascular physiologic processes: ventricular function: ventricular function, left research

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Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Radiology

VOLUME: 245

Page Numbers: 196-205

Journal Abbreviation:

ISSN: 0033-8419

DAY: 3

MONTH: Oct

YEAR: 2007

Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 401260

Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Keywords Mesh Terms:

KEYWORDS: Ventricular Function, Left

MESH TERMS: physiology

Chemical & Substance for Abstract: Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain. Information

Substance Name: Meglumine

Registry Number: 6284-40-8

Grant and Affiliation Information for Injection of adeno-associated viral vector encoding vascular endothelial growth factor gene in infarcted swine myocardium: MR measurements of left ventricular function and strain.

AFFILIATION: Department of Radiology, University of California San Francisco, 513 Parnassus Ave, HSW 207 B, San Francisco, CA 94134-0628, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01HL07295

ACRONYM: HL

MEDLINETA: Radiology

REFSOURCE: Radiology. 2007 Oct;245(1):1-2

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