Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery.

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Abstract Text:

Britt-Marie ,Ulla ,Anders Hyltander,David Ljungman,Kent Lundholm,Britt-Marie ,Ulla ,Anders Hyltander,David Ljungman,Kent Lundholm,

Previous studies have provided conflicting conclusions concerning the efficacy of improving protein balance in patients by standard intravenous nutrition [TPN (total parenteral nutrition)], which is either explained by suboptimal nutritional regimens or insensitive clinical methods. The aim of the present study was therefore to evaluate the effects on the initiation of translation of skeletal muscle proteins by standard overnight TPN. A total of 12 patients who underwent standard surgery were included. TPN was provided as an all-in-one treatment by constant infusion [0.16 gN.kg(-1) of body weight.day(-1) (30 kcal.kg(-1) of body weight.day(-1))]. Saline-infused patients served as controls. Rectus abdominis muscle biopsies were taken at the time of the operation. The phosphorylation state of the proteins for initiation of translation was quantified. Plasma glucose, and serum insulin, glycerol, triacylglycerols (triglycerides) and NEFAs (non-esterified fatty acids; 'free fatty acids') were not significantly altered during TPN infusion, whereas total plasma amino acids increased, as shown by increases in methionine, phenylalanine, threonine, alanine, arginine, aspartic acid, glycine and histidine (P<0.05). Overnight TPN increased the formation of active eIF4G-eIF4E (where eIF is eukaryotic-initiation factor) complexes (P<0.05), whereas the inhibitory complex 4E-BP1 (eIF4E-binding protein)-eIF4E was moderately decreased (P<0.06). TPN increased the amount of the most phosphorylated form of 4E-BP1 (P<0.05), and increased the amount (P<0.04) and phosphorylation (P<0.01) of p70(S6K) (70 kDa ribosomal protein S6 kinase). In conclusion, an overnight pre-operative constant infusion of standard TPN altered initiation factor complexes, indicating activation of the initiation of protein translation in rectus abdominis muscle in the presence of increased plasma amino acid levels, but without a concomitant increase in energy substrates and insulin. In contrast with our results from previous studies, the methodology used in the present study appears to be more sensitive in reflecting directional changes in human muscle protein synthesis compared with traditional methods, particularly based on measurements of amino acid flux.

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Publishing Authors By Initials

BM ,U ,A Hyltander,D Ljungman,K Lundholm,BM ,U ,A Hyltander,D Ljungman,K Lundholm,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical science (London, England : 1979)

VOLUME: 114

Page Numbers: 603-10

Journal Abbreviation: Clin. Sci.

ISSN: 1470-8736

DAY: 1

MONTH: May

YEAR: 2008

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905731

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery.

AFFILIATION: Department of Surgery, Surgical Metabolic Research Laboratory at Lundberg Laboratory for Cancer Research, Sahlgrenska University Hospital, SE413 45 Göteborg, Sweden.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Clin Sci (Lond)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery Related Publications

• Bacterial contamination of cotton and cotton dust and effects on the lung.
• Culture of tubercle bacilli from specimens of gastric juice.
• Daily physical-rest activities in relation to nutritional state, metabolism, and quality of life in cancer patients with progressive cachexia.
• Endotoxin levels in farming: absence of symptoms despite high exposure levels.
• Exposure to nitrous oxide in delivery suites at six Swedish hospitals.
• Fractures around the neck of the femur.
• Growth associated proteins in tumor cells and stroma related to disease progression of colon cancer accounting for tumor tissue PGE2 content.
• Initiation factors for translation of proteins in the rectus abdominis muscle from patients on overnight standard parenteral nutrition before surgery.
• Interactions of cytokines in the host.
• Internal fixation of hip fractures with a new lag screw.
• Isolation of radiopure plasma and hepatic albumin in acute phase conditions.
• Nutritional problems in trauma.
• Preoperative treatment with a non-steroidal anti-inflammatory drug (NSAID) increases tumor tissue infiltration of seemingly activated immune cells in colorectal cancer.
• Survival and erythropoietin receptor protein in tumours from patients randomly treated with rhEPO for palliative care.
• The effect of DFP on respiration, blood pressure and muscular function in the rabbit.
• The mechanism of the vasodilator effect of adrenaline. I. Effect on skeletal muscle vessels.
• [Not Available.]
• [Not Available.]