Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine.

Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Research Abstract Details 

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Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Abstract Text:

Guo-Du Wang,Xi-Yu Wang,Hong-Zhen Hu,Sumei Liu,Na Gao,Xiucai Fang,Yun Xia,Jackie D Wood,

ATP is a putative inhibitory neurotransmitter responsible for inhibitory junction potentials (IJPs) at neuromuscular junctions (IJPs) in the intestine. This study tested the hypothesis that the purinergic P2Y(1) receptor subtype mediates the IJPs. IJPs were evoked by focal electrical stimulation in the myenteric plexus and recorded with "sharp" intracellular microelectrodes in the circular muscle coat. Stimulation evoked three categories of IJPs: 1) purely purinergic IJPs, 2) partially purinergic IJPs, and 3) nonpurinergic IJPs. Purely purinergic IJPs were suppressed by the selective P2Y(1) purinergic receptor antagonist MRS2179. Purely purinergic IJPs comprised 26% of the IJPs. Partially purinergic IJPs (72% of the IJPs) consisted of a component that was abolished by MRS2179 and a second unaffected component. The MRS2179-insensitive component was suppressed or abolished by inhibition of formation of nitric oxide by N(omega)-nitro-l-arginine methyl ester (l-NAME) in some, but not all, IJPs. An unidentified neurotransmitter, different from nitric oxide, mediated the second component in these cases. Nonpurinergic IJPs were a small third category (4%) of IJPs that were abolished by l-NAME and unaffected by MRS2179. Exogenous application of ATP evoked IJP-like hyperpolarizing responses, which were blocked by MRS2179. Application of apamin, which suppresses opening of small-conductance Ca(2+)-operated K(+) channels in the muscle, decreased the amplitude of the purinergic IJPs and the amplitude of IJP-like responses to ATP. The results support ATP as a neurotransmitter for IJPs in the intestine and are consistent with the hypothesis that the P2Y(1) purinergic receptor subtype mediates the action of ATP.

Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Publishing Authors By Initials

GD Wang,XY Wang,HZ Hu,S Liu,N Gao,X Fang,Y Xia,JD Wood,

For similar heterocyclic compounds: heterocyclic compounds, 2-ring: quinazolines: tetrodotoxin research abstracts see: heterocyclic compounds: heterocyclic compounds, 2-ring: quinazolines: tetrodotoxin research

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Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 292

Page Numbers: G1483-9

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 22

MONTH: 02

YEAR: 2007

Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Information

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LANGUAGE: eng

NlmUniqueID: 100901227

Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Keywords Mesh Terms:

KEYWORDS: Tetrodotoxin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine. Information

Substance Name: Nitric Oxide Synthase

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Inhibitory neuromuscular transmission mediated by the P2Y1 purinergic receptor in guinea pig small intestine.

AFFILIATION: Dept. of Physiology and Cell Biology, The Ohio State Univ., College of Medicine and Public Health, 304 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK-37238

ACRONYM: DK

MEDLINETA: Am J Physiol Gastrointest Live

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