[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells]

[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells] Research Abstract Details 

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[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells] Abstract Text:

Xin-jun Lei,Ai-qun Ma,Yu-tao Xi,Wei Zhang,Yan Yao,Yuan Du,

AIM: To investigate the expression of high-conductance Ca2+-activated potassium channel (MaxiK channel) mRNA and protein during human monocyte-derived macrophage differentiation into foam cells and to study its function in foam cell formation. METHODS: Human peripheral blood monocytes were isolated from healthy male volunteers by density gradient centrifugation and then by adherent method. The obtained monocytes were cultured for 5 days to differentiate into macrophages. Based on establishment of human macrophage-derived foam cells model, the expression of MaxiK channel alpha-subunit was investigated by immunocytochemical staining, RT-PCR and Western blot. Furthermore, the effect of Paxilline, a MaxiK channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein (OxLDL) was studied. RESULTS: After the macrophages were co-incubated with 30 mg/L OxLDL at 37 degrees C for 60 hours, the cellular volume obviously enlarged and many red lipid granules were deposited in cytoplasm. The total amount of cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) in cells markedly increased and the ratio of CE/TC rose from (14.437+/-6.781)% to (57.946+/-3.507)% (n=7, P<0.05). However, the expression of MaxiK channel alpha-subunit had no significant change (P<0.05). Paxilline (5 micromol/L and 10 micromol/L) markedly reduced the content of TC, FC and CE in macrophages and the ratio of CE/TC decreased to (41.217+/-5.584)% (5 micromol/L Paxilline) and (18.017+/-11.559)% (10 micromol/L Paxilline), respectively (n=7, P<0.05). Meanwhile, the red lipid granules deposited in the cytoplasm of macrophages also decreased. CONCLUSION: Blocking MaxiK channel can inhibit human monocyte-derived macrophage to be differentiated into foam cells.

[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells] Publishing Authors By Initials

XJ Lei,AQ Ma,YT Xi,W Zhang,Y Yao,Y Du,

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[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 22

Page Numbers: 310-3

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 28

MONTH: May

YEAR: 2006

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LANGUAGE: chi

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AFFILIATION: Department of Cardiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Cardiovascular Ion Channelopathy Laboratory, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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