Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions.

Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Research Abstract Details 

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Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Abstract Text:

R Kii,K Sakai,A Sano,K Yonezawa,E Hashimoto,H Yamamura,

Guanyl-5'-yl imidodiphosphate (Gpp(NH)p), a nucleotide phosphohydrolase-resistant analog of GTP, caused inhibitory and stimulatory effects on the basal adenylate cyclase activity of rat synaptosomal fractions when manganese was present in the assay mixture, whereas the nucleotide caused only a stimulatory effect when magnesium was employed. In the presence of manganese, the inhibitory and stimulatory effects of Gpp(NH)p could be seen at around concentrations of 10(-7) M and 10(-4) M Gpp(NH)p, respectively. The inhibitory and stimulatory effects of Gpp(NH)p were both antagonized competitively by GTP; these effects of the analog were the opposite of those observed with GTP, which was stimulatory and inhibitory for fat call adenylate cyclase at 10(-7) M and 10(-4) M, respectively (Yamamura, H., Lad, P.M., and Rodbell, M. (1977) J. Biol. Chem. 252, 7964--7966). The degree of inhibition by Gpp(NH)p did not depend on the concentration of manganese nor on the addition of ethylene glycol bis(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid.

Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Publishing Authors By Initials

R Kii,K Sakai,A Sano,K Yonezawa,E Hashimoto,H Yamamura,

For similar cells: cellular structures: subcellular fractions: synaptosomes research abstracts see: cells: cellular structures: subcellular fractions: synaptosomes research

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Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 267-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1980

Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Keywords Mesh Terms:

KEYWORDS: Synaptosomes

MESH TERMS: enzymology

Chemical & Substance for Abstract: Inhibitory and stimulatory effects of guanyl-5'-yl imidodiphosphate on the adenylate cyclase activity of rat synaptosomal fractions. Information

Substance Name: Adenylate Cyclase

Registry Number: EC 4.6.1.1

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Country: JAPAN

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MEDLINETA: J Biochem

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