Inhibition of U snRNP assembly by a virus-encoded proteinase.

Inhibition of U snRNP assembly by a virus-encoded proteinase. Research Abstract Details 

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Inhibition of U snRNP assembly by a virus-encoded proteinase. Abstract Text:

Laura L Almstead,Peter Sarnow,

It has been proposed that defects in the assembly of spliceosomal uridine-rich small nuclear ribonucleoprotein (U snRNP) complexes could account for the death of motor neurons in spinal muscular atrophy (SMA). We discovered that infection of cultured cells with poliovirus results in the specific cleavage of the host factor Gemin3 by a virus-encoded proteinase, 2A(pro). Gemin3 is a component of the macromolecular SMN complex that mediates assembly of U snRNP complexes by aiding the heptameric oligomerization of Sm proteins onto U snRNAs. Using in vitro Sm core assembly assays, we found that lowering the intracellular amounts of Gemin3 by either poliovirus infection or small interfering RNA (siRNA)-mediated knockdown of Gemin3 resulted in reduced assembly of U snRNPs. Immunofluorescence analyses revealed a specific redistribution of Sm proteins from the nucleoplasm to the cytoplasmic periphery of the nucleus in poliovirus-infected cells. We propose that defects in U snRNP assembly may be shared features of SMA and poliomyelitis.

Inhibition of U snRNP assembly by a virus-encoded proteinase. Publishing Authors By Initials

LL Almstead,P Sarnow,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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MEDLINE DATE:

Inhibition of U snRNP assembly by a virus-encoded proteinase. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genes & development

VOLUME: 21

Page Numbers: 1086-97

Journal Abbreviation:

ISSN: 0890-9369

DAY: 1

MONTH: May

YEAR: 2007

Inhibition of U snRNP assembly by a virus-encoded proteinase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8711660

Inhibition of U snRNP assembly by a virus-encoded proteinase. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Inhibition of U snRNP assembly by a virus-encoded proteinase. Information

Substance Name: picornain 2A, Picornavirus

Registry Number: EC 3.4.22.29

Grant and Affiliation Information for Inhibition of U snRNP assembly by a virus-encoded proteinase.

AFFILIATION: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM07276

ACRONYM: GM

MEDLINETA: Genes Dev

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