Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript.

Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript. Research Abstract Details 

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Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript. Abstract Text:

Kevin F Bryant,Donald M Coen,Kevin F Bryant,Donald M Coen,

Many viruses regulate gene expression, both globally and specifically, to achieve maximal rates of replication. During herpes simplex virus 1 infection, translation of the DNA polymerase (Pol) catalytic subunit is inefficient relative to other proteins of the same temporal class (D. R. Yager, A. I. Marcy, and D. M. Coen., J. Virol. 64:2217-2225, 1990). To investigate the mechanisms involved in the inefficient translation of Pol and to determine whether this inefficient translation could affect viral replication, we performed a mutagenic analysis of the 5' end of the pol transcript. We found that a short sequence ( approximately 55 bases) in the 5' leader of the transcript is both necessary and sufficient to inhibit translation in rabbit reticulocyte lysates and sufficient to inhibit reporter gene translation in transfected cells. RNase structure mapping experiments indicated that the inhibitory element adopts a structure that contains regions of a double-stranded nature, which may interfere with ribosomal loading and/or scanning. Pol accumulated to approximately 2- to 3-fold-higher levels per mRNA in cells infected with a mutant virus containing a deletion of the approximately 55-base inhibitory element than in cells infected with a control virus containing this element. Additionally, the mutant virus replicated less efficiently than the control virus. These results suggest that the inhibitory element regulates Pol translation during infection and that its inhibition of Pol translation is beneficial for viral replication.

Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript. Publishing Authors By Initials

KF Bryant,DM Coen,KF Bryant,DM Coen,

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Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 82

Page Numbers: 77-85

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 24

MONTH: 10

YEAR: 2007

Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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Grant and Affiliation Information for Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript.

AFFILIATION: Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Ave., Boston, MA 02115, USA.

Country: United States

AGENCY: United States NCI

GRANT: T32 CA09031

ACRONYM: CA

MEDLINETA: J Virol

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