Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells.

Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells. Research Abstract Details 

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Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells. Abstract Text:

Yu Fan,You-Li Zhang,Ying Wu,Wei Zhang,Yin-Huan Wang,Zhao-Ming Cheng,Hua Li,Yu Fan,You-Li Zhang,Ying Wu,Wei Zhang,Yin-Huan Wang,Zhao-Ming Cheng,Hua Li,

AIM: To investigate the roles and mechanism of signal transducer and activator of transcription 3 (STAT3) in invasion of human colon cancer cells by RNA interference. METHODS: Small interfering RNA (siRNA) targeting Signal transducer and activator of transcription 3 (STAT3) was transfected into HT29 colon cancer cells. STAT3 protein level and DNA-binding activity of STAT3 was evaluated by western blotting and electrophoretic mobility shift assay (EMSA), respectively. We studied the anchorage-independent growth using colony formation in soft agar, and invasion using the boyden chamber model, anoikis using DNA fragmentation assay and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL), respectively. Western blot assay was used to observe the protein expression of Bcl-xL and survivin in colon cancer HT29 cells. RESULTS: RNA interference (RNAi) mediated by siRNA leads to suppression of STAT3 expression in colon cancer cell lines. Suppression of STAT3 expression by siRNA could inhibit anchorage-independent growth, and invasion ability, and induces anoikis in the colon cancer cell line HT29. It has been shown that knockdown of STAT3 expression by siRNA results in a reduction in expression of Bcl-xL and survivin in HT29 cells. CONCLUSION: These results suggest that STAT3 siRNA can inhibit the invasion ability of colon cancer cells through inducing anoikis, which antiapoptotic genes survivin and Bcl-xL contribute to regulation of anoikis. These studies indicate STAT3 siRNA could be a useful therapeutic tool for the treatment of colon cancer.

Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells. Publishing Authors By Initials

Y Fan,YL Zhang,Y Wu,W Zhang,YH Wang,ZM Cheng,H Li,Y Fan,YL Zhang,Y Wu,W Zhang,YH Wang,ZM Cheng,H Li,

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Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: World journal of gastroenterology : WJG

VOLUME: 14

Page Numbers: 428-34

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 21

MONTH: Jan

YEAR: 2008

Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells. Information

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LANGUAGE: eng

NlmUniqueID: 100883448

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Grant and Affiliation Information for Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells.

AFFILIATION: Department of Gastroenterology, Affiliated Hospital, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China. zjyouli@yahoo.com.cn.

Country: China

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MEDLINETA: World J Gastroenterol

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