Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes.

Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Research Abstract Details 

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Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Abstract Text:

K Nakamura,T Sasada,H Sono,J Yodoi,

The CD4 molecule acts as a receptor for class II MHC molecules to stabilize Ag recognition by the TCR and as a high affinity receptor for HIV-1. In this study, we investigated the effect of oxidative stress on the level of CD4 expression on cultured peripheral blood T lymphocytes (PBL blasts). As previously reported, we observed that the surface CD4 was down-regulated by PMA. Unexpectedly, treatment of PBL blasts with hydrogen peroxide (H2O2) or a sulfhydryl oxidative reagent, diamide, almost completely inhibited PMA-induced CD4 down-regulation, although these oxidants per se did not affect the level of CD4 expression. We next assessed the serine phosphorylation of CD4, which is reported to be an indispensable process for PMA-induced CD4 endocytosis. PMA could induce the serine phosphorylation even in the presence of oxidants. We also found that these oxidants had an additive effect on PMA-induced dissociation between CD4 and p56(lck), which is known to be another necessary step for CD4 endocytosis. These results indicate that in T cells, oxidants inhibit protein kinase C-mediated CD4 down-regulation due to perturbing a signaling process other than the above two steps, implying that oxidative stress may tune the functions of CD4+ T cells and their susceptibility to HIV-1 through the control of CD4 expression.

Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Publishing Authors By Initials

K Nakamura,T Sasada,H Sono,J Yodoi,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-tyrosine kinases: src-family kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-tyrosine kinases: src-family kinases research

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Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 157

Page Numbers: 5339-49

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 15

MONTH: Dec

YEAR: 1996

Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Information

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LANGUAGE: eng

NlmUniqueID: 2985117

Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Keywords Mesh Terms:

KEYWORDS: src-Family Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes. Information

Substance Name: Protein Kinase C

Registry Number: EC 2.7.11.13

Grant and Affiliation Information for Inhibition of protein kinase C-mediated CD4 down-regulation by oxidative stress in T lymphocytes.

AFFILIATION: Department of Biological Responses, Institute for Virus Research, Kyoto University, Japan.

Country: UNITED STATES

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MEDLINETA: J Immunol

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