Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine.
Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine. Research Abstract Details
Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine. Abstract Text:
We investigated the role of nitric oxide (NO) in pacemaker activity and signal mechanisms in cultured interstitial cells of Cajal (ICC) of the mouse small intestine using whole cell patch-clamp techniques at 30 degrees C. ICC generated pacemaker potential in the current clamp mode and pacemaker currents at a holding potential of -70 mV. (+/-)-S-nitroso-N-acetylpenicillamine (SNAP; a NO donor) produced membrane hyperpolarization and inhibited the amplitude and frequency of the pacemaker currents, and increased resting currents in the outward direction. These effects were blocked by the use of glibenclamide (an ATP-sensitive K(+) channel blocker), but not by the use of 5-hydroxydecanoic acid (a mitochondrial ATP-sensitive K(+) channel blocker). Pretreatment with ODQ (a guanylate cyclase inhibitor) almost blocked the NO-induced effects. The use of cell-permeable 8-bromo-cyclic GMP also mimicked the action of SNAP. However, the use of KT-5823 (a protein kinase G inhibitor) did not block the NO-induced effects. Spontaneous [Ca(2+)](i) oscillations in ICC were inhibited by the treatment of SNAP, as seen in recordings of intracellular Ca(2+) ([Ca(2+)](i)). These results suggest that NO inhibits pacemaker activity by the activation of ATP-sensitive K(+) channels via a cyclic GMP dependent mechanism in ICC, and the activation of ATP-sensitive K(+) channels mediates the inhibition of spontaneous [Ca(2+)](i) oscillations.
Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine. Publishing Authors By Initials
Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Naunyn-Schmiedeberg's archives of pharmacology
Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine. Information
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LANGUAGE: eng
NlmUniqueID: 326264
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Grant and Affiliation Information for Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K(+) channels in cultured interstitial cells of Cajal from the mouse small intestine.
AFFILIATION: Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, South Korea.
Country: Germany
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MEDLINETA: Naunyn Schmiedebergs Arch Phar
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