[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats]

[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats] Research Abstract Details 

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[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats] Abstract Text:

Guang-yi Wang,Shi-hui Zhu,Hong-tai Tang,Sheng-de Ge,Zhao-fan Xia,

OBJECTIVE: To study whether nimodipine, a dihydropyridine-type calcium channel blocker, can inhibit the production of interleukin-1beta(IL-1beta), interleukin-6(IL-6) by Kupffer cells(KC) and down-regulate its level of plasma after severe burn injury. METHODS: KC of normal rats were isolated with portal vein catheter, intrahepatic digestion and density gradient centrifugation. Intracellular calcium concentration ([Ca2+]i) in individual KC after stimulated with postburn serum was assessed fluorometrically with microspectrofluorometer. Level of IL-1beta and IL-6 in the supernatant of KC cultured with postburn serum were detected by enzyme-linked immunosorbent assay(ELISA). SD rats underwent 30% total body surface area(TBSA) full thickness burn 6 hours later, KCs was isolated and their mRNA were extracted. Level of IL-1beta mRNA and IL-6 mRNA were detected by ribonuclease protection assay(RPA). Levels of plasma IL-1beta and IL-6 were also detected. Role of nimodipine on above-mentioned effects were observed. RESULTS: Compared with that of control group, levels of [Ca2+]i of KCs and IL-1beta and IL-6 supernatant in burn group increased significantly(all P<0.01). At present of 1 micromol/L nimodipine, however, the [Ca2+]i, IL-1beta, IL-6 values decreased significantly(all P<0.01). The level of plasma cytokines and KC mRNA in burn group also increased significantly. After intravenously injection with nimodipine (40 microg x kg(-1) x h(-1)), the numerical values decreased significantly(all P<0.01). CONCLUSION: Kupffer cells of rats are activated to secret IL-1beta and IL-6 after severe burn injury and this process is realized through calcium ion signal transduction channel. Nimodipine can inhibit IL-1beta and IL-6 production of KC by preventing its mRNA transcription, down-regulating its level of plasma.

[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats] Publishing Authors By Initials

GY Wang,SH Zhu,HT Tang,SD Ge,ZF Xia,

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[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese cr

VOLUME: 15

Page Numbers: 210-2

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ISSN: 1003-0603

DAY: 14

MONTH: Apr

YEAR: 2003

[Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats] Information

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LANGUAGE: chi

NlmUniqueID: 9887521

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AFFILIATION: The Burn Department, Changhai Hospital, Shanghai 200433, China.

Country: China

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MEDLINETA: Zhongguo Wei Zhong Bing Ji Jiu

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