Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions.

Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. Research Abstract Details 

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Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. Abstract Text:

Takahisa Takino,Hiromi Saeki,Hisashi Miyamori,Tomoya Kudo,Hiroshi Sato,Takahisa Takino,Hiromi Saeki,Hisashi Miyamori,Tomoya Kudo,Hiroshi Sato,

Membrane-type 1 matrix metalloproteinase (MT1-MMP) has been implicated in tumor invasion and metastasis. We previously reported that extracellular matrix degradation by MT1-MMP regulates cell migration via modulating sustained integrin-mediated signals. In this study, MT1-MMP-expressing cells were plated onto fibronectin-coated plates and monitored for cell-matrix adhesion formation and fibronectin degradation. The fibronectin was degraded and removed in line with the cell migration track. The migrating cells showed a polarized morphology and were in contact with the edge of fibronectin through the leading edge, in which cell-matrix adhesions are concentrated. Expression of MT1-MMP targeted to cell-matrix adhesions by fusing with the focal adhesion targeting (FAT) domain of focal adhesion kinase (FAK) promoted the initial fibronectin lysis at the cell periphery immediately after adhesion. These results suggest that fibronectin is degraded by MT1-MMP located at cell-matrix adhesions, which are concentrated at the leading edge of the migrating cells. To inhibit MT1-MMP at cell-matrix adhesion, the dominant negative form of MT1-MMP (MT1-Pex) was targeted to the cell-matrix adhesion by fusing with the FAT domain (MT1-Pex-FAT). MT1-Pex-FAT accumulated at cell-matrix adhesions and inhibited fibronectin degradation as well as FAK phosphorylation more effectively than parental MT1-Pex. MT1-Pex-FAT was also shown to suppress the invasion of tumor cells into three-dimensional collagen gel more strongly than MT1-Pex. These results suggest that MT1-MMP-mediated extracellular matrix lysis at cell-matrix adhesions induces the establishment of cell polarity, which facilitates cell-matrix adhesion turnover and subsequent cell migration. This model highlights the role of MT1-MMP at the leading edge of migrating cells.

Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. Publishing Authors By Initials

T Takino,H Saeki,H Miyamori,T Kudo,H Sato,T Takino,H Saeki,H Miyamori,T Kudo,H Sato,

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Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cancer research

VOLUME: 67

Page Numbers: 11621-9

Journal Abbreviation: Cancer Res.

ISSN: 1538-7445

DAY: 15

MONTH: Dec

YEAR: 2007

Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. Information

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LANGUAGE: eng

NlmUniqueID: 2984705

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Grant and Affiliation Information for Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions.

AFFILIATION: Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan. ttakino@kenroku.kanazawa-u.ac.jp

Country: United States

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MEDLINETA: Cancer Res

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