Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin.

Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Research Abstract Details 

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Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Abstract Text:

T Hirano,H Yamada,T Miyazaki,

The carbohydrate requirement for cell adhesion of aggregation-competent cells of Dictyostelium discoideum has been examined by use of a selective glycosylation inhibitor of N-glycosyl protein, tunicamycin (TM). TM completely inhibited EDTA-stable cell adhesion and glycosylation of some membrane glycoproteins in aggregation-competent cells of D. discoideum (Yamada, H., et al. (1982) J. Biochem. 92, 399-406). The present study showed that the inhibition of EDTA-stable cell adhesion by TM was prevented significantly when the cells were treated with TM in the presence of a protease inhibitor, leupeptin (LP), whereas the inhibition of glycosylation by TM was not prevented. The cell extract of aggregation-competent cells contained acid proteases, and LP strongly inhibited acid protease from D. discoideum in vitro. On analysis by SDS-polyacrylamide gel electrophoresis (PAGE), many protein bands present in the membrane fraction of control cells disappeared or decreased on TM treatment of the cells in the absence of LP, however, some of these proteins were restored when the cells were treated with TM in the presence of LP. These results strongly support an idea that EDTA-stable cell adhesion characteristic to aggregation-competent cells is mediated by glycoproteins with asparagine-linked carbohydrate. However, the requirement for the carbohydrate moiety of the glycoprotein in cell adhesion appears to be indirect in that it acts to protect the protein moiety from proteolytic degradation.

Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Publishing Authors By Initials

T Hirano,H Yamada,T Miyazaki,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleosides: tunicamycin research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleosides: tunicamycin research

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Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 1249-57

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1983

Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Keywords Mesh Terms:

KEYWORDS: Tunicamycin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Inhibition of cell adhesion in Dictyostelium discoideum by tunicamycin is prevented by leupeptin. Information

Substance Name: Aspartic Endopeptidases

Registry Number: EC 3.4.23.-

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Country: JAPAN

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MEDLINETA: J Biochem

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