Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes.

Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes. Research Abstract Details 

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Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes. Abstract Text:

Chronic skin exposure to UV radiation manifests in a score of biochemical events, DNA damage and mutations which can potentially cause skin cancer. The ubiquitin proteasome pathway controls the degradation of a majority of regulatory eukaryotic proteins including those which play a key role in tumorigenesis. SCF(betaTrCP) E3 ubiquitin ligases mediate ubiquitination and proteasomal degradation of phosphorylated substrates that play a key role in signal transduction. Activation of several signaling pathways involved in tumorigenesis was shown to elevate expression and activity of beta-TrCP1/2. In this study, we established and characterized human neonatal foreskin keratinocytes, rendered immortal by retroviral introduction of human telomerase reverse transcriptase (hTERT). These skin hTERT immortalized normal keratinocytes (STINKs) maintain characteristic traits of keratinocytes, such as expression of keratins, cytoplasmic localization of basonuclin and susceptibility to high concentration of calcium. We analyzed the response of STINKs to UVB radiation and its classical markers, such as p53 and nuclear factor (NF)-kappaB. We also demonstrate that inhibition of beta-TrCP2 function, by induction of dominant negative beta-TrCP2 (beta-TrCP2(DeltaN)), accentuates UVB induced apoptosis, and this phenomenon is independent of NF-kappaB and p53 pathways.

Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes. Publishing Authors By Initials

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MEDLINE DATE: 2008 Mar-Apr

Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Photochemistry and photobiology

VOLUME: 84

Page Numbers: 376-81

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 15

MONTH: 01

YEAR: 2008

Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Inhibition of beta-TrCP Function Potentiates UVB-induced Apoptosis in hTERT-immortalized Normal Human Keratinocytes.

AFFILIATION: Department of Dermatology and Paul P. Carbone Comprehensive Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.

Country: United States

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MEDLINETA: Photochem Photobiol

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