Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone.

Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone. Research Abstract Details 

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Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone. Abstract Text:

Chao-Bin Xue,Wan-Chun Luo,Lin Jiang,Xian-Ye Xie,Ting Xiao,Lei Yan,Chao-Bin Xue,Wan-Chun Luo,Lin Jiang,Xian-Ye Xie,Ting Xiao,Lei Yan,

Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the present study, the kinetic assay in air-saturated solutions and the kinetic behavior of PO from Pieris rapae (Lepidoptera) larvae in the oxidation of L-tyrosine (a monophenol) and L-DOPA (l-3, 4-dihydroxyphenylalanine) (a diphenol) was studied. The inhibitory effects of 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone (3-H-4-MBT) on the monophenolase and diphenolase activities of PO were also studied. The results show that 3-H-4-MBT can inhibit both the monophenolase and diphenolase activities of PO. The lag period of L-tyrosine oxidation catalyzed by the enzyme was obviously lengthened and the steady-state activities of the enzyme sharply decreased. The inhibitor was found to be noncompetitively reversible with a K I (K I = K IS) of 0.30 micromol/L and an estimated IC50 of 0.14 +/- 0.02 micromol/L for monophenolase and 0.26 +/- 0.04 micromol/L for diphenolase. In the time course of the oxidation of L-DOPA catalyzed by the enzyme in the presence of different concentrations of 3-H-4-MBT, the rate decreased with increasing time until a straight line was approached. The microscopic rate constants for the reaction of 3-H-4-MBT with the enzyme were determined.

Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone. Publishing Authors By Initials

CB Xue,WC Luo,L Jiang,XY Xie,T Xiao,L Yan,CB Xue,WC Luo,L Jiang,XY Xie,T Xiao,L Yan,

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Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied biochemistry and biotechnology

VOLUME: 143

Page Numbers: 101-14

Journal Abbreviation: Appl. Biochem. Biotechnol.

ISSN: 0273-2289

DAY: 20

MONTH: Nov

YEAR: 2007

Inhibition kinetics of cabbage butterfly (Pieris rapae L.) larvae phenoloxidase activity by 3-hydroxy-4-methoxybenzaldehyde thiosemicarbazone. Information

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LANGUAGE: eng

NlmUniqueID: 8208561

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AFFILIATION: College of Plant Protection, Key Laboratory of Pesticide Toxicology and Application Technique, Shandong Agricultural University, Shandong Tai'an, People's Republic of China.

Country: United States

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MEDLINETA: Appl Biochem Biotechnol

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